Abstract
These studies were designed to investigate the lymph absorption of a lipid emulsion in rats prefed different long-term high-fat diets. Particular emphasis was placed on the consequences of endogenous fatty acid alteration on the lymph recovery of two labeled fatty acids. Male Wistar rats were fed a standard diet (LF) containing 3.5 g/100 g fat or high-fat diets containing 15 g/100 g sunflower oil (HSFO), menhaden oil (HMO) or medium-chain triglyceride oil (HMCT) for 4 wk. The lymph was collected for 3 h before and after the intraduodenal infusion of a 90 micromol lipid emulsion (30 micromol monopalmitin, 30 micromol oleic acid, 25 micromol linoleic acid, 5 micromol arachidonic acid) labeled with [3H] oleic (OA) and [14C] arachidonic (AA) acids. The [3H] OA and [14C] AA lymph recoveries were measured and the lymph samples were tested for fatty acid, phospholipid and triglyceride content. Prefeeding an HSFO or HMO diet led to a 65 or 32% greater total lymph fatty acid output, respectively, compared with rats prefed the LF diet. In rats prefed both the HSFO and HMO diets, lymph fatty acid characteristics provided evidence of a dilution of exogenous fatty acids coming from the emulsion by endogenous fatty acids. In rats prefed the ...Continue Reading
References
Dec 1, 1976·Canadian Journal of Biochemistry·I M Yousef, M M Fisher
Aug 1, 1977·The Journal of Clinical Investigation·C M Mansbach
Apr 1, 1975·The Journal of Clinical Investigation·A Gangl, R K Ockner
Mar 4, 1992·Biochimica Et Biophysica Acta·G R HerzbergK M Keough
Apr 23, 1992·Biochimica Et Biophysica Acta·R Coleman, K Rahman
Aug 1, 1992·The Journal of Nutrition·C PaveroH Carlier
Jan 1, 1991·Reproduction, Nutrition, Development·H CarlierC Caselli
May 1, 1991·Experimental Physiology·A Bernard, H Carlier
Apr 24, 1991·Biochimica Et Biophysica Acta·R KnoxC M Mansbach
Feb 1, 1985·The Journal of Nutrition·I S ChenG V Vahouny
Dec 20, 1973·Biochimica Et Biophysica Acta·J B RodgersR J O'Brien
Jun 1, 1972·The Journal of Clinical Investigation·E R SchiffJ M Dietschy
Jul 1, 1967·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·B K ShrivastavaW J Simmonds
Nov 1, 1969·The Journal of Clinical Investigation·R K OcknerK J Isselbacher
Feb 1, 1966·The Journal of Clinical Investigation·N J GreenbergerK J Isselbacher
Apr 4, 1967·Biochimica Et Biophysica Acta·S A HyunC R Treadwell
Jan 1, 1984·The Journal of Clinical Investigation·G M PattonS J Robins
Mar 1, 1993·Gut·A P Jenkins, R P Thompson