PMID: 108201Aug 1, 1978

Long-term antibody synthesis in vitro. VI. Anti-allotype sera as probes of clonal products in affinity maturation

E Conway de MacarioS Landucci-Tosi


A new experimental system is described for measuring the allotypic product of rabbit B cells during long-lasting in vitro antibody responses. The immunoenzymatic assays described allow determination of several parameters mapping in different regions of the same molecule, which can be measured and combined to yield a multidimensional picture of the time-course dynamics of antibody synthesis. The rabbit immune system responding to Escherichia coli beta-D-galactosidase was sample and disassembled by (a) culturing lymph node microfragments and (b) sorting out from among all anti-enzyme antibodies only those activating a mutant enzyme, AMEF, which bore the b4 or b9 allotype. A considerable simplification of the response was achieved in the microcultures as documented by cultures of heterozygous cells which produced only one allotype and by the fact that each culture showed a distinctive pattern when antibody titre, association constant, heterogeneity index, L-chain type, and k-chain allotype were considered together. This array of patterns was not an artifact but the result of disassembling a representative sample of the rabbit immune system into small components, since the b4/b9 ratio obtained by averaging the results of all cultur...Continue Reading

Related Concepts

Kappa allotype b4, rabbits
Antibodies, Bacterial
Antibody Avidity
Antibody Formation
Clone Cells
Alkalescens-Dispar Group
Immune Sera
Peroxidase-Antiperoxidase Complex Technique
Immunoglobulin Allotypes

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