Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct

Cytotherapy
Stéphanie PasquetJean-Pierre Monassier

Abstract

Starting from experimental data proposing hematopoietic stem cells as candidates for cardiac repair, we postulated that human peripheral blood (PB) CD34+ cells mobilized by hematopoietic growth-factor (G-CSF) would contain cell subpopulations capable of regenerating post-ischemic myocardial damages. In a phase I clinical assay enrolling seven patients with acute myocardial infarct, we directly delivered to the injured myocardium autologous PB CD34+ cells previously mobilized by G-CSF, collected by leukapheresis and purified by immunoselection. In parallel, we looked for the eventual presence of cardiomyocytic and endothelial progenitor cells in leukapheresis products of these patients and controls, using flow cytometry, reverse transcription-quantitative (RTQ)-polymerase chain reaction (PCR), cell cultures and immunofluorescence analyzes. The whole clinical process was feasible and safe. All patients were alive at an average follow-up of 49 months (range 24-76 months). Improvement of heart function parameters became obvious from the third month following cell reinjection. Left ventricular ejection fraction values progressively and dramatically increased with time, associated with PetScan demonstration of myocardial structure re...Continue Reading

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Citations

Jul 31, 2012·American Journal of Physiology. Cell Physiology·Michalis MastriTechung Lee
Dec 2, 2011·Biological & Pharmaceutical Bulletin·So-Hyun ParkJong-Hyuk Sung
May 16, 2014·PloS One·Ulrike RitzAlexander Hofmann
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Apr 17, 2020·Stem Cell Reviews and Reports·Philippe Hénon
May 30, 2019·Bioengineering·Minh Nguyen Tuyet Le, Kouichi Hasegawa
Nov 9, 2018·Frontiers in Cell and Developmental Biology·Yuning Hou, Chunying Li

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