Long-Term Continuous Cultivation of Clostridium beijerinckii in a Two-Stage Chemostat with On-Line Solvent Removal.

Applied and Environmental Microbiology
J R GapesA Friedl

Abstract

A two-stage continuous cultivation experiment with Clostridium beijerinckii NRRL B592 is described. This strain maintained its ability to produce neutral solvents (acetone, n-butanol, and ethanol) at an overall dilution rate of 0.13 h(sup-1) and achieved an average overall solvent concentration of 9.27 g/liter and an overall solvent productivity of 1.24 g/liter/h for more than 100 overall retention times. The experiment was performed without pH control on a semisynthetic medium containing yeast extract, and product inhibition was the limiting factor. Solid carrier material was present in both stages, and the solvent productivity in both stages was similar. A membrane evaporation module integrated into the recirculation loop of a second-stage bioreactor after 2,166 h increased solvent productivity and improved the yield of solvents by about 40%. The membrane reduced the concentration of solvents, which would otherwise inhibit the fermentation. Additionally, the integrated membrane evaporation dampened metabolic oscillations, which are characteristic of continuous cultivation of clostridia. It was also demonstrated that a moderate concentration buildup (approximately 30% of bioreactor inflow) caused by water flux through the memb...Continue Reading

References

Jan 1, 1979·Advances in Applied Microbiology·S Ogata, M Hongo
Jan 1, 1989·Biotechnology & Genetic Engineering Reviews·I S Maddox
Dec 1, 1986·Microbiological Reviews·D T Jones, D R Woods
Dec 1, 1952·Journal of Bacteriology·A KUTZENOK, M ASCHNER
Jan 1, 1959·Applied Microbiology·R K FINN, J E NOWREY
Dec 1, 1993·Applied and Environmental Microbiology·E R Kashket, Z Y Cao
Apr 1, 1983·Biotechnology and Bioengineering·I M Jöbses, J A Roels
Feb 5, 1988·Biotechnology and Bioengineering·S R RofflerC R Wilke
Nov 1, 1989·Biotechnology and Bioengineering·R ShuklaK K Sirkar
Apr 25, 1994·Biotechnology and Bioengineering·Q Geng, C H Park

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Citations

May 17, 2011·Applied Microbiology and Biotechnology·Shrikant A SurvaseTom Granström
Feb 19, 2014·Applied Microbiology and Biotechnology·Chuang XueJian-Xin Sun
Nov 20, 2016·Biotechnology and Bioengineering·Dongming XieQuinn Zhu
May 10, 2020·Applied and Environmental Microbiology·François WaselsNicolas Lopes Ferreira
Aug 4, 2016·PloS One·Edward A Buehler, Ali Mesbah
Apr 4, 2017·Biotechnology for Biofuels·Sylvia GildemynLargus T Angenent
Aug 18, 2016·Applied Microbiology and Biotechnology·Sang-Hyun LeeKyoung Heon Kim
Feb 12, 2017·Biotechnology Progress·Victoria OutramAdam P Harvey
Sep 8, 2020·Journal of Industrial Microbiology & Biotechnology·Charlotte Anne VeesStefan Pflügl
Apr 11, 2018·BMC Genomics·Hadrien Máté de GérandoNicolas Lopes Ferreira
Nov 17, 2020·Frontiers in Microbiology·Constantinos PatiniosServé W M Kengen

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