PMID: 2492964Mar 1, 1989Paper

Long-term cryogenic storage of purified adult human islets of Langerhans

Diabetes
N M KnetemanP E Lacy

Abstract

Reliable high-recovery human islet storage would facilitate tissue matching, organ sharing, and immune manipulation of donor islets and prospective diabetic recipients. Collagenase-isolated, Ficoll-purified pancreatic islets (median 21,000, 15% of total islet yield) from eight cadaver pancreases were cultured in vitro for 24 h, equilibrated in three steps with dimethyl sulfoxide (DMSO) to a 2-M concentration, supercooled, nucleated, and cooled at 0.25 degree C/min to -40 degrees C before storage at -196 degrees C for 44.25 +/- 8.75 days. Rewarming at 200 degrees C/min and removal of DMSO with 0.75 M sucrose preceded 48 h of culture and retesting. Recovery postthaw by microscope count on duplicate aliquots was 94.2 +/- 3.5% of prefreeze counts and by triplicate assay of extractable insulin was 90.0 +/- 22.3% on day 0 and 74.1 +/- 12.6% after a 48-h culture. Nonfrozen islets increased basal insulin secretion 7.7 +/- 2.8 times after stimulation with 300 mg/dl glucose in perifusion, whereas islets frozen-thawed and cultured 48 h increased 6.2 +/- 0.8 times (NS). Peak stimulated insulin release was 0.92 +/- 0.14 microU.islet-1.min-1 before storage and 0.73 +/- 0.14 microU.islet-1.min-1 (79% of control, NS) after freeze-thaw and a 48...Continue Reading

Citations

May 7, 1997·Transfusion Science·A Penfornis
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A PermuttM Mueckler
Dec 1, 1993·The Anatomical Record·M M el-NaggarM Tahir
Apr 14, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Di Liu, Feng Pan
May 2, 2015·Diabetologia·Jocelyn E Manning FoxPatrick E MacDonald
Sep 6, 2017·Biopreservation and Biobanking·Takahiro YamanakaShinichi Hochi
May 7, 2003·Transfusion Medicine Reviews·Lennart LögdbergChristopher D Hillyer
May 18, 2021·Journal of Tissue Engineering and Regenerative Medicine·Kenyu Nakayama-IwatsukiShinichi Hochi

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