Long term culture of cells derived from mouse blastocysts.

Differentiation; Research in Biological Diversity
M I Sherman

Abstract

The development of mouse blastocysts in primary culture has been followed for up to two months. The trophectoderm layer of the blastocyst gives rise to a monolayer of trophoblast cells; cells resembling both ectoplacental cone cells and primary giant cells are observed. The former can transform to giant cells, presumably secondary trophoblast, after several days in culture. Giant trophoblast cells are evident in the culture for much longer than the normal gestation period. Under the culture conditions described, the proportion of blastocysts showing substantial inner cell mass (ICM) proliferation in vitro is higher than that noted in previous studies. The ICM clumps develop into either egg cylinder-like structures, or, more commonly, into spherical, fluid-filled vesicles. The vesicles, which resemble yolk sac morphologically and biochemically [10, 11], continue to enlarge in size during several weeks of culture. The vesicles are attached to the underlying trophoblast monolayers by a stalk. Cells appear to migrate from this stalk out along the culture dish. The result after two to four weeks of culture is the appearance of a mixed monolayer containing a variety of different cell types. Secondary cultures of blastocyst cells have...Continue Reading

References

Oct 7, 1966·Annals of the New York Academy of Sciences·R B Gwatkin
Oct 1, 1968·Journal of Cellular Physiology·S A Aaronson, G J Todaro
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M I Sherman, N J Chew
Dec 1, 1972·Experimental Cell Research·M I Sherman
Jul 1, 1973·Developmental Biology·K E Bell, M I Sherman
Jul 15, 1970·American Journal of Obstetrics and Gynecology·Z KorenS J Behrman
May 14, 1971·Nature·Y C Hsu
Oct 1, 1970·Journal of Reproduction and Fertility·T M Menke, A McLaren
Nov 1, 1968·Journal of Reproduction and Fertility·W K Whitten, J D Biggers
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT
Jul 1, 1959·Virology·R DULBECCO, G FREEMAN

❮ Previous
Next ❯

Citations

Jan 1, 1981·Anatomy and Embryology·E B Ilgren
Dec 1, 1975·Developmental Biology·D S Salomon, M I Sherman
Sep 1, 1978·Developmental Biology·S B Atienza-Samols, M I Sherman
Sep 1, 1980·Developmental Biology·S B Atienza-SamolsM I Sherman
Jun 1, 1981·Developmental Biology·J Schindler, M I Sherman
Jul 1, 1983·Placenta·E B Ilgren
May 30, 2002·Journal of Anatomy·R L Gardner
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A Rizzino, C Crowley
Aug 1, 1989·The Journal of Cell Biology·S J FisherC H Damsky
Oct 1, 1978·Archives of Biochemistry and Biophysics·C D Little, R L Church
Jul 1, 1979·Experimental Cell Research·A Rizzino, M I Sherman
Aug 1, 1978·Experimental Cell Research·S L Abreu, R L Brinster
Mar 15, 1979·Experimental Cell Research·A M JettenJ P Poon
Jan 1, 1980·Journal of Supramolecular Structure·A RizzinoH Rizzino
Dec 30, 1982·The Journal of Experimental Zoology·E J BlakeM I Sherman
May 1, 1983·Archives of Biochemistry and Biophysics·M J TocciJ J Monahan
Jun 1, 1980·Differentiation; Research in Biological Diversity·K O YanagisawaK Moriwaki
Jan 1, 1980·Differentiation; Research in Biological Diversity·B R CullenJ J Monahan
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Vania JanuarRichard Saffery
Nov 10, 1977·Nature·L R WudlN Hillman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.