PMID: 7019042May 1, 1981Paper

Long-term culture of epithelial cells from the normal rat colon

In Vitro
D P Chopra, K Y Yeh

Abstract

Serial passage cultures of colonic epithelial cells from young rats have been maintained for more than 6 months in Eagle's minimum essential medium buffered with HEPES (25 mM) and supplemented with 2.5% fetal bovine serum, 0.5 micrograms/ml insulin, 5.0 micrograms/ml transferrin, and antibiotics. The cells proliferated in this medium with a population doubling time of approximately 53 h. The cells retained differentiated morphology as evidenced by secretory activity and the presence of secretory granules, microvilli, tonofilaments, and desmosomal junctions. Further, cells at the fourth passage had normal karyotypes with 42 chromosomes and exhibited anchorage dependent growth. High concentrations of fetal bovine serum (10 to 15%) exerted toxic effects on the colonic epithelial cell cultures.

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Citations

Sep 1, 1986·Cell Biology and Toxicology·J H ResauC Newkirk
Jul 1, 1983·Digestive Diseases and Sciences·A TeranoK J Ivey
Aug 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D W RattnerW Silen
Jul 8, 2009·Stem Cells and Development·Dharam P ChopraGraham C Parker
May 25, 2002·In Vitro Cellular & Developmental Biology. Animal·Bertrand Kaeffer
Dec 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C BrantingJ Rafter
Feb 1, 1987·Journal of Cellular Physiology·D P ChopraK M Siddiqui
Apr 1, 1989·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·A M BrownB J Baum
Feb 1, 1995·Differentiation; Research in Biological Diversity·D P ChopraL V Reddy
Jan 1, 1987·Differentiation; Research in Biological Diversity·M KedingerP Simon-Assmann
Dec 5, 2006·Methods in Enzymology·Matthew Bjerknes, Hazel Cheng
Apr 1, 1994·International Journal of Colorectal Disease·R FontiY Sambuy
Mar 11, 2005·Alternatives to Laboratory Animals : ATLA·Sascha BirknerWolfram Föllmann

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