Long-term culture of functional hepatocytes on chemically modified collagen gels.

Cytotechnology
N ShimbaraA Ichihara

Abstract

For long-term maintenance of functional hepatocytes in primary culture, a new culture system with chemically modified type-I collagen gel was developed. Isolated hepatocytes spread as flat cells and rapidly lost their viability and functions when cultured on native collagen gel. In contrast, they survived for several weeks when cultured on collagen gels that had been modified by treatment with sodium-borohydride (NaBH(4)) or by digestion with pepsin, which resulted in destruction of crosslinking of collagen fibers and marked decrease in meachanical strength of the gels. These long-lived cells were round and aggregated and maintained high levels of various differentiated liver functions including albumin secretion and activities of tyrosine aminotransferase and P450. Moreover on collagen gels modified by treatment with NaBH(4) or pepsin, the cell showed less DNA synthesis in response to mitogenic stimulation than cells cultures on gel containing native collagen. Interestingly, crosslinking of these chemically modified gels with D-ribose resulted in changes in various phenotypes of hepatocytes cultures on them including shape, longevity, and functions expressed when the cells were cultured on native collagen gel, suggesting that ...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J T EmermanS Nandi
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 31, 1991·Biochemical and Biophysical Research Communications·A KumatoriA Ichihara
Apr 16, 1990·Biochemical and Biophysical Research Communications·C NodaA Ichihara
Dec 1, 1988·Experimental Cell Research·T NakamuraA Ichihara
Mar 1, 1988·Journal of Cellular Physiology·E G SchuetzP S Guzelian
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H C IsomJ Mummaw
Mar 1, 1981·The Journal of Cell Biology·H K KleinmanG R Martin
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T D SargentJ Bonner

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Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·Pi-Chao Wang, Toshiaki Takezawa
Apr 17, 1997·Biochemical and Biophysical Research Communications·S M D'AmbrosioF M Robertson
Sep 1, 1998·Journal of Gastroenterology and Hepatology·Ryoichi AwataToshihiro Sugiyama

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