PMID: 8583835Sep 15, 1995Paper

Long-term culture of monoclonal human T lymphocytes: models for immunosenescence?

Mechanisms of Ageing and Development
M AdibzadehG Pawelec

Abstract

Human monoclonal T lymphocyte populations maintained in long-term culture by intermittent reactivation via the antigen receptor and supplied with exogenous interleukin 2 manifest finite proliferative lifespans. T lymphocytes cloned from mature peripheral T cells of adult donors were constantly lost from the time point of their first isolation up to an estimated maximum of 80 population doublings (PD) for the longest lived. T lymphocytes cloned from T cell progenitors in bone marrow, on the other hand, survived for a maximum of ca. 100 PD. One facet of the functional capacity of cells derived from these two different sources was assessed by measuring their autocrine proliferation after mitogenic stimulation. For a majority of T cell clones (TCC), autocrine proliferative capacity decreased as a function of culture age, becoming absent by 50 PD for adult-derived-TCC and by 70 PD for bone marrow-derived TCC, thereby clearly occurring prior to the end of the proliferative life spans of the clones. Limiting dilution frequency analysis showed that the number of autocrine proliferative precursors within these monoclonal populations declined with age, paralleling loss of autocrine proliferative capacity in the 'bulk' clones. Of a variet...Continue Reading

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Citations

Oct 1, 1998·Age·E D Bernstein, D M Murasko
Oct 25, 1996·Mechanisms of Ageing and Development·M AdibzadehG Pawelec
Feb 13, 2001·Mechanisms of Ageing and Development·J D McLeod
Aug 21, 2001·Mechanisms of Ageing and Development·G PawelecT Fülöp
Mar 6, 2003·Mechanisms of Ageing and Development·Rita B Effros
Dec 21, 2002·Neurobiology of Aging·L A PanossianR B Effros
Oct 24, 2002·Human Immunology·Urban J ScheuringArgyrios N Theofilopoulos
Feb 26, 2000·Vaccine·R B Effros
Feb 26, 2000·Vaccine·G PawelecA Engel
Jun 6, 1998·Experimental Gerontology·N BoucherF Schächter
Aug 5, 1999·Experimental Gerontology·G PawelecA Engel
Oct 26, 1999·Experimental Gerontology·M PotestioG C Romano
Mar 16, 2000·Microbes and Infection·R B Effros
Feb 26, 1998·Immunological Reviews·G PawelecM Adibzadeh
Apr 13, 2005·Nephron. Clinical Practice·Sagrario SorianoPedro Aljama
Feb 14, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Ke ZhangPengfei Tu
Sep 28, 2014·Cellular Immunology·Yoshihiro KushidaMasamichi Hosono
May 11, 2005·Immunological Reviews·Hui-Chen HsuJohn D Mountz
May 11, 2005·Immunological Reviews·Rita B EffrosJanice Man
Mar 21, 2003·Immunology and Allergy Clinics of North America·Graham PawelecAnders Wikby
Jan 24, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P TrzonkowskiK J Wood
Jan 10, 2013·Current Opinion in Immunology·Joel CrespoWeiping Zou
Jul 30, 2020·Journal of Leukocyte Biology·Aimee M MerinoFrank Cichocki
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MigliaccioM Nabholz
Feb 15, 2019·Frontiers in Immunology·Graham Pawelec
Jul 4, 2018·Frontiers in Immunology·Nato TeteloshviliBart-Jan Kroesen
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D VoehringerH Pircher

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