Long-Term Culture of Organotypic Hippocampal Slice from Old 3xTg-AD Mouse: An ex vivo Model of Alzheimer's Disease

Psychiatry Investigation
Sooah JangEosu Kim

Abstract

Conventional methods for organotypic hippocampal tissue slice culture (OHSC) have shown several disadvantages or limitations regarding age of animals used, duration of culture and difficulty using neurodegenerative models. Therefore, we tried to establish OHSC from old 3xTg-Alzheimer's disease (AD) mice for longer period (over 4 weeks) and to validate utility of this system as a valid platform for translational neuroscience of AD. OHSC was performed with old 3xTg-AD mice (12-14 months), old wild type mice (12-14 months) and young 3xTg-AD mice (2-4 months) using serum-free medium for 4 weeks. Hippocampal structure was evaluated by 4', 6-diamidino-2-phenylindole (DAPI) intensity and neuronal metabolism was measured by Alamarblue assay. Pathologic characteristics of AD were also investigated; β-amyloid levels by ELISA, amyloid plaque deposition by Thioflavin-S staining, and glial activation by immunohistochemistry. Following 4-week culture in serum-free media, hippocampal cells and layers were well preserved in cultured slices from old AD mice as was in those from young AD and old wild type mice. On the contrary, excessive regression of total visible cells was observed in conventional serum-containing medium regardless of genotype...Continue Reading

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Citations

Dec 14, 2018·Current Alzheimer Research·Christian Humpel
May 10, 2019·International Journal of Molecular Sciences·Kamal M YakoubValentina Di Pietro
Dec 20, 2018·Journal of Cellular and Molecular Medicine·Ciqing YangJuntang Lin
Feb 8, 2019·Scientific Reports·César R Romero-LeguizamónKristi A Kohlmeier
Jul 30, 2019·Frontiers in Aging Neuroscience·Mariana ToricelliHudson Sousa Buck
Jul 23, 2020·Nature·Charles H LiRichard A Young
Jul 3, 2021·Advanced Functional Materials·Michael L LovettDavid L Kaplan
Jan 5, 2022·Reviews in the Neurosciences·Alla B SalminaSergey N Illarioshkin

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Methods Mentioned

BETA
transgenic
dissection
ELISA
protein assay

Software Mentioned

ImageJ
SPSS

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