Long-term durability, tissue regeneration and neo-organ growth during skeletal maturation with a neo-bladder augmentation construct

Regenerative Medicine
Manuel J JayoTimothy A Bertram

Abstract

To comparatively evaluate bladder regeneration following 80% cystectomy and augmentation using a synthetic biopolymer with autologous urothelial and smooth muscle cells (autologous neo-bladder augmentation construct [construct]) or autotransplantation of native bladder (reimplanted native urinary bladder [reimplant]) in canines. Voiding function, urodynamic assessment and neo-organ capacity-to-body-weight ratio (C:BW) were assessed longitudinally for a total of 24 months following trigone-sparing augmentation cystoplasty in juvenile canines. Within 30 days postimplantation, hematology and urinalysis returned to baseline. Constructs and reimplants yielded neo-organs with statistically equivalent urodynamics and histology. Linear regression analysis of C:BW showed that constructs regained baseline slope and continued to adapt with animal growth. Constructs and reimplants regained and maintained native bladder histology by 3 months, capacity at 3-6 months and compliance by 12-24 months. Furthermore, construct C:BW demonstrated the ability of regenerated bladder to respond to growth regulation.

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Citations

May 9, 2013·Current Urology Reports·Blake W Palmer, Bradley P Kropp
Dec 6, 2011·Tissue Engineering. Part a·Joydeep BasuJohn W Ludlow
Nov 11, 2011·Tissue Engineering. Part B, Reviews·John W LudlowTimothy A Bertram
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Methods Mentioned

BETA
biopsy
biopsies

Software Mentioned

Excel
JMP

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