Long-term effect of gene therapy for chronic ischemic myocardium using platelet-derived endothelial cell growth factor in dogs

The Journal of Gene Medicine
Wei LiAkio Ihaya

Abstract

We previously reported the 2-week benefits of platelet-derived endothelial cell growth factor (PD-ECGF) gene therapy in chronically ischemic myocardium. However, the long-term effects and safety using this gene have not been reported. Chronic myocardial ischemia was created in 24 dogs by stenosing the origin of the left anterior descending coronary artery (LAD) using an ameroid constrictor. Two weeks later, the PD-ECGF gene, the LacZ gene, or saline was infused directly into the myocardium in the LAD area. The myocardial blood volume and myocardial function were examined prior to ischemia, immediately before gene injection, and for 6 months following injection, and then the organs were harvested for histological and molecular examination. PD-ECGF gene treatment significantly attenuated endocardial infarction at 6 months. Myocardial blood volume and myocardial function decreased in all three groups after ameroid implantation, but recovered after 2 weeks in the PD-ECGF-treated group, and maintained a higher level of function during the examination period. Histological analysis demonstrated that angiogenesis and arteriogenesis occurred after PD-ECGF gene treatment. There was a decreased expression of the pro-apoptotic proteins, ac...Continue Reading

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Citations

Nov 26, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Thiagarajan HemalathaRengarajulu Puvanakrishnan
Jun 18, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Thiagarajan HemalathaRengarajulu Puvanakrishnan
May 13, 2009·Medicinal Research Reviews·Annelies BronckaersSandra Liekens
Jan 12, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Carolyn A HolladayAbhay Pandit
Oct 8, 2014·Circulation Research·Wei LiRoy L Silverstein
Jun 24, 2017·Heart Failure Reviews·Hemalatha ThiagarajanAnbukkarasi Kaliyaperumal

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