Long-Term Effects of Dietary Protein and Branched-Chain Amino Acids on Metabolism and Inflammation in Mice

Nutrients
Wei-Chieh MuRyan W Grant

Abstract

Aging is the main factor involved in the onset of degenerative diseases. Dietary protein restriction has been shown to increase the lifespan of rodents and improve metabolic phenotype. Branched-chain amino acids (BCAA) can act as nutrient signals that increase the lifespan of mice after prolonged supplementation. It remains unclear whether the combination of protein restriction and BCAA supplementation improves metabolic and immunological profiles during aging. Here, we investigated how dietary protein levels and BCAA supplementation impact metabolism and immune profile during a 12-month intervention in adult male C57BL/6J mice. We found that protein restriction improved insulin tolerance and increased hepatic fibroblast growth factor 21 mRNA, circulating interleukin (IL)-5 concentration, and thermogenic uncoupling protein 1 in subcutaneous white fat. Surprisingly, BCAA supplementation conditionally increased body weight, lean mass, and fat mass, and deteriorated insulin intolerance during protein restriction, but not during protein sufficiency. BCAA also induced pro-inflammatory gene expression in visceral adipose tissue under both normal and low protein conditions. These results suggest that dietary protein levels and BCAA su...Continue Reading

References

Jul 5, 1996·Science·R A Miller
Apr 15, 2000·Physiology & Behavior·B D WhiteR J Martin
Mar 26, 2003·Biochemical Society Transactions·C Franceschi, M Bonafè
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Giovanni PasseriClaudio Franceschi
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Laura HaynesSusan L Swain
Dec 14, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shinobu NishitaniIchiro Sonaka
Nov 23, 2006·Mechanisms of Ageing and Development·Claudio FranceschiStefano Salvioli
Nov 23, 2006·Mechanisms of Ageing and Development·Sonya VastoCalogero Caruso
Jan 4, 2007·The Journal of Pathology·A L GruverG D Sempowski
Sep 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dayong WuSimin Nikbin Meydani
Oct 5, 2007·The New England Journal of Medicine·Kristin L NicholEelko Hak
Apr 9, 2008·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Mauro ZamboniVincenzo Di Francesco
Aug 9, 2008·Endocrinology·Tamer CoskunAlexei Kharitonenkov
Sep 2, 2009·Blood·Hyunwon YangVishwa Deep Dixit
Jul 30, 2010·Current Opinion in Immunology·Albert C ShawJanet M Lord
Nov 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carey N LumengRaymond L Yung
Jan 12, 2012·The Biochemical Journal·Ana Luísa De Sousa-CoelhoDiego Haro
Feb 4, 2012·Genes & Development·Matthew J PotthoffDavid J Mangelsdorf
Feb 10, 2012·Cellular Immunology·Lorena Alvarez-RodríguezVíctor Manuel Martínez-Taboada
Mar 6, 2012·Cell·Varman T Samuel, Gerald I Shulman
Dec 12, 2012·Annual Review of Immunology·Scott N MuellerWilliam R Heath
Feb 9, 2013·Endocrinology and Metabolism Clinics of North America·Gary J Schwartz
Apr 20, 2013·Nature Immunology·Jörg J Goronzy, Cornelia M Weyand
Jun 12, 2013·Cell·Carlos López-OtínGuido Kroemer
Oct 9, 2013·Nature Medicine·Matthew Harms, Patrick Seale
Jun 6, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Thomas LaegerChristopher D Morrison
Aug 19, 2014·The Journal of Clinical Investigation·Thomas LaegerChristopher D Morrison
Sep 23, 2014·Archives of Pharmacal Research·Min Hi ParkHae Young Chung
Oct 23, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·David G Le CouteurStephen J Simpson
Nov 26, 2014·PharmacoEconomics·Francesco CoricaGiulio Marchesini
Jul 30, 2015·European Journal of Immunology·Felix M WensveenBojan Polić

❮ Previous
Next ❯

Citations

Apr 23, 2020·Nutrients·Paulina Markowiak-Kopeć, Katarzyna Śliżewska
Dec 11, 2019·Nutrients·Emma C E MeessenMaarten R Soeters
Sep 10, 2020·Science Signaling·Carlos GuijasGary Siuzdak
Mar 4, 2020·Mechanisms of Ageing and Development·Bushra AminRenã A S Robinson
Jul 16, 2021·Translational Medicine of Aging·Reji Babygirija, Dudley W Lamming
Dec 4, 2020·Aging and Disease·Alexey MoskalevCalogero Caruso
Sep 21, 2021·Critical Reviews in Food Science and Nutrition·Elżbieta SupruniukAdrian Chabowski

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
flow cytometry
PCR
ELISA

Software Mentioned

Mascot Daemon
Ingenuity Pathway Analysis
SAS
FCS Express
Ingenuity Pathway Analysis ( IPA )

Related Concepts

Related Feeds

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

Aging-Associated Metabolic Disorders

Age is associated with many metabolic disorders including cardiovascular diseases, type 2 diabetes, stroke and heart disease. The mediators in aging process have been suggested to play a part in the cellular processes responsible for these metabolic disorders. Here is the latest research on aging-associated metabolic disorders.