PMID: 7028907Nov 1, 1981Paper

Long-term effects of leucine and arginine on B-cell function of cultivated pancreatic rat islets

The Journal of Endocrinology
G Schäfer, H Schatz

Abstract

Leucine and arginine are well-known stimulators of insulin release, but they exert different actions on insulin biosynthesis in short-term incubations of isolated islets. In order to study their long-term effects of the B-cell, isolated rat islets were cultivated for 40 h in medium containing 0.5 or 3 mg glucose/ml supplemented with L-leucine (10 mmol/l) or L-arginine (10 mmol/l). After the culture the islets were incubated for 3 h with 2 mg glucose/ml without addition of the respective amino acid. Insulin biosynthesis was estimated from incorporation of [3H]phenylalanine or [3H]leucine into the (pro)insulin fraction of the islet proteins during this incubation. At the low concentrations of glucose, the leucine-cultivate islets released much more insulin than the control islets during cultures as well as during subsequent incubation. At both glucose concentrations cultivated together with this amino acid resulted in an enhanced insulin biosynthesis. The insulin-releasing effect of arginine with 0.5 mg glucose/ml was not as marked as that observed with an equimolar concentration of leucine. Islets cultivated with arginine showed no response to glucose during the incubation after culture. Hormone synthesis was found to be inhibit...Continue Reading

Citations

Jul 1, 1983·Molecular and Cellular Endocrinology·G SchäferH Schatz
Jun 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·L M KarabatasJ C Basabe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.