Long-term elevation of cyclooxygenase-2, but not lipoxygenase, in regions synaptically distant from spreading depression

The Journal of Comparative Neurology
A O CaggianoR P Kraig

Abstract

Eicosanoids, produced from arachidonic acid by cyclooxygenases (COXs) and lipoxygenases (LIPOXs), are involved in numerous brain processes. To explore if brief and noninjurious stimuli chronically alter expression of these enzymes, we examined the induction of COX-2 and LIPOX expression following unilateral neocortical spreading depression (SD). Expression was examined over time and in regions not experiencing SD (hippocampus) but synaptically connected to the site of stimulation (cortex). One hundred six male Wistar rats had SD induced via microinjection of 0.5 M KCl (0.5 M NaCl for sham) into left parietal cortex every 9 minutes for 1 or 3 hours. One hour before SD some animals received dexamethasone (Dex), mepacrine (Mep), indomethacin (Indo), nordihydroguaiaretic acid (Ndga), phenylephrine (Pe), sodium nitroprusside (Snp) with Pe, or N omega-nitro-L-arginine methyl ester (Lnam). Animals survived for 0, 3, or 6 hours, or 1, 2, 3, 7, 14, 21, or 28 days. Brains were processed immunohistochemically for COX-2 and LIPOX, and the optical density (OD) of the left and right cortex, dentate gyrus (DG), CA3, and CA1 immunoreactivity (IR) were measured. Induction was expressed as the log of left divided by right side OD for each region...Continue Reading

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Citations

Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M NakayamaS H Graham
May 6, 2004·Journal of Neuroscience Methods·R E HulseR P Kraig
Apr 15, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Phillip E KunklerRichard P Kraig
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Jan 15, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A O Caggiano, R P Kraig
Aug 21, 2014·Nutritional Neuroscience·Claudia MarschollekAli Gorji
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Nov 13, 2007·Brain, Behavior, and Immunity·Kenneth I Strauss
Mar 15, 2011·Brain Research·Miriam S DomowiczNancy B Schwartz

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