Long-term exposure of the freshwater shrimp Macrobrachium olfersii to elevated salinity: effects on gill (Na+,K+)-ATPase alpha-subunit expression and K+-phosphatase activity

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
N N MendonçaRosa P M Furriel

Abstract

The kinetic properties of a microsomal gill (Na+,K+)-ATPase from the freshwater shrimp, Macrobrachium olfersii, acclimated to 21 per thousand salinity for 10 days were investigated using the substrate p-nitrophenylphosphate. The enzyme hydrolyzed this substrate obeying cooperative kinetics at a rate of 123.6+/-4.9 U mg-1 and K0.5=1.31+/-0.05 mmol L-1. Stimulation of K+-phosphatase activity by magnesium (Vmax=125.3+/-7.5 U mg-1; K0.5=2.09+/-0.06 mmol L-1), potassium (Vmax=134.2+/-6.7 U mg-1; K0.5=1.33+/-0.06 mmol L-1) and ammonium ions (Vmax=130.1+/-5.9 U mg-1; K0.5=11.4+/-0.5 mmol L-1) was also cooperative. While orthovanadate abolished p-nitrophenylphosphatase activity, ouabain inhibition reached 80% (KI=304.9+/-18.3 micromol L-1). The kinetic parameters estimated differ significantly from those for freshwater-acclimated shrimps, suggesting expression of different isoenzymes during salinity adaptation. Despite the approximately 2-fold reduction in K+-phosphatase specific activity, Western blotting analysis revealed similar alpha-subunit expression in gill tissue from shrimps acclimated to 21 per thousand salinity or fresh water, although expression of phosphate-hydrolyzing enzymes other than (Na+,K+)-ATPase was stimulated by h...Continue Reading

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Citations

Feb 14, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nesrine Boudour-BouchekerCatherine Lorin-Nebel
Sep 12, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tania Zenteno-SavínMarcelo Hermes-Lima
Jan 21, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Malson N LucenaFrancisco A Leone
Dec 10, 2013·TheScientificWorldJournal·Valerie VranovaPavel Formanek
May 25, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·L C F SantosR P M Furriel
Dec 4, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·I IntanaiN M Whiteley
Dec 23, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·N M BelliR P M Furriel
Jun 19, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Douglas C MasuiFrancisco A Leone

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