Long-term exposure to oxidized low-density lipoprotein enhances tumor necrosis factor-alpha-stimulated endothelial adhesiveness of monocytes by activating superoxide generation and redox-sensitive pathways

Free Radical Biology & Medicine
Jaw-Wen ChenShing-Jong Lin

Abstract

This study was conducted to investigate the role of oxidized low-density lipoprotein (LDL) in monocyte/mononuclear cell (MNC) activation during atherogenesis. First, the activity of MNCs was studied in patients with coronary artery disease (CAD). Compared to normal subjects, phorbol 12-myristate 13-acetate (PMA)-stimulated reactive oxygen species (ROS) production and the adhesiveness to endothelial cells were increased in MNCs from CAD patients. After 24-h coculture with oxidized LDL, ROS elaboration of MNCs was significantly increased in CAD patients. It was further correlated to the endothelial adhesiveness of MNCs (r = 0.561, P < 0.05). Secondly, in an in vitro model for long-term, direct effects of oxidized LDL on murine monocytoid cells (MMCs), oxidized LDL, but not native LDL, induced ROS production of MMCs in a time-dependent manner up to a 4-day coincubation (261% elevation, P < 0.05). Four-day coincubation with ox-LDL enhanced cytoplasmic IkappaB phosphorylation and nuclear factor kappa B (NF-kappaB) translocation and increased endothelial adhesiveness of MMCs. The long-term exposure to oxidized LDL also significantly enhanced tumor necrosis factor-alpha (TNF-alpha)-stimulated ROS production and endothelial adhesivenes...Continue Reading

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Citations

Nov 11, 2006·Current Opinion in Lipidology·Ngoc-Anh Le
Jun 12, 2012·Journal of Biomedicine & Biotechnology·Jung-Tung LiuYung-Hsiang Chen
Aug 22, 2017·Frontiers in Endocrinology·Undurti N Das
Jan 30, 2018·Journal of Biomaterials Science. Polymer Edition·Zhu-Le WangHui-Qi Xie
May 24, 2019·Frontiers in Pharmacology·Gulcin TezcanSvetlana F Khaiboullina
Dec 6, 2020·The Science of the Total Environment·Kaihang XuJun Wang
Oct 30, 2007·Journal of Bioscience and Bioengineering·Chi-Chao YinKuang-Tse Huang

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