Long-term exposure to retinoic acid induces the expression of IRK1 channels in HERG channel-endowed neuroblastoma cells

Biochemical and Biophysical Research Communications
A ArcangeliM Olivotto

Abstract

The modulation of inward K+ conductances was studied during neuronal differentiation of human SH-SY5Y neuroblastoma cells. Under standard culture conditions, these cells express the herg gene, and the HERG current is the main inward K+ current regulating their Vrest. After 10-20 days exposure to Retinoic Acid (RA), SH-SY5Y cells showed, in addition to HERG currents, a novel current characterized by inward rectification, dependence on the extracellular K+ concentration, and blockade by Cs+ and Ba2+, the main features of the IRK1 current. The appearance of this current is accompanied by a strong hyperpolarisation of Vrest. RT-PCR experiments confirmed that a transcript of the IRK1 (Kir 2.1) gene actually appears in SH-SY5Y cells treated for 10-20 days with RA. On the whole, data here presented demonstrate that RA-induced neuronal differentiation of neuroblastoma cells is accompanied by the switch from a HERG-driven to a IRK1-driven control of Vrest, similarly to what happens in normal differentiating neurons; however, in tumor cells, this switch does not imply the abolition of HERG channel expression.

References

Dec 1, 1991·Cancer Metastasis Reviews·C J Thiele
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S PåhlmanE Ortoft
Jul 1, 1994·Acta Physiologica Scandinavica·S Johansson
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·J W Warmke, B Ganetzky
Apr 1, 1996·The American Journal of Physiology·S JohanssonU Kahl
Feb 1, 1997·Circulation Research·R S WymoreI S Cohen
Jun 1, 1997·The Journal of Physiology·N ChiesaE Wanke
Jul 17, 1997·Nature·T V McDonaldG I Fishman

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Citations

Jun 7, 2013·Tissue Engineering. Part a·Sarah SundelacruzDavid L Kaplan
May 11, 2005·Progress in Neurobiology·Michelle A Lane, Sarah J Bailey
Sep 5, 2015·BioMed Research International·Elena LastraioliAnnarosa Arcangeli
Jun 13, 2013·Current Opinion in Genetics & Development·Emily A Bates
May 15, 2007·Trends in Cell Biology·Michael Levin
Jan 18, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazunori WatanabeTakashi Ohtsuki
Dec 29, 2013·Journal of Neurophysiology·Nicholas D Vesprini, Gaynor E Spencer
Jun 30, 2009·Stem Cell Reviews and Reports·Sarah SundelacruzDavid L Kaplan
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Jürgen R. Schwarz, Christiane K. Bauer

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