Long-term exposure to retrovirally expressed granulocyte-colony-stimulating factor induces a nonneoplastic granulocytic and progenitor cell hyperplasia without tissue damage in mice.

The Journal of Clinical Investigation
J M ChangG R Johnson

Abstract

Murine marrow cells infected with a retroviral vector (MPZen) bearing a granulocyte-colony-stimulating factor (G-CSF) cDNA insert were transplanted into lethally irradiated recipients to study the effects of autocrine production of G-CSF in normal hemopoietic cells. Most animals remained healthy with no evidence of tissue damage throughout the observation period (4-30 wk) despite high circulating G-CSF levels (range 2,000-26,000,000 U/ml). A dramatic neutrophilic granulocytosis was observed in all hemopoietic tissues with neutrophilic infiltration occurring in the lung and liver. Spleen, peritoneal, and peripheral blood cellularity increased approximately three-, two-, and eightfold, respectively, but total bone marrow cell counts remained unchanged. Progenitor cell numbers granulocyte-macrophage colony-forming cell (GM-CFC), granulocyte colony-forming cell (G-CFC), burst-forming unit-erythroid (BFU-E), colony-forming unit-erythroid (CFU-E) and mixed colony-forming cells (Mix-CFC) were elevated between 10-100-fold in the spleen, peritoneal cavity, and peripheral blood, but were unaffected or slightly depressed in the marrow. No tumors developed in syngeneic recipients transplanted with bone marrow or spleen cells from such mice...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G R Johnson, D Metcalf
May 1, 1987·Analytical Biochemistry·D D Bowtell
Feb 1, 1988·The Journal of Experimental Medicine·A MuraguchiT Kishimoto
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TsuchiyaS Nagata
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A M CohenL M Souza
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K WelteM A Moore
Feb 6, 1985·Nature·M B Sporn, A B Roberts
Dec 1, 1970·Virology·W P RoweJ W Hartley
Oct 1, 1980·The Journal of Experimental Medicine·T M DexterD Metcalf

❮ Previous
Next ❯

Citations

Jan 1, 1990·International Journal of Cell Cloning·D Metcalf
Apr 16, 1991·Biochimica Et Biophysica Acta·J M Adams, S Cory
Apr 6, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
May 25, 2010·Nature Reviews. Cancer·Donald Metcalf
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S W ChungJ Petersen
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·X JiangC Eaves
Oct 1, 1992·The Journal of Experimental Medicine·R G HawleyT S Hawley
May 1, 1990·British Journal of Haematology·D Metcalf
Jul 24, 2010·Molecular Cancer·María M CaffarelCristina Sánchez
Dec 26, 2006·Circulation Journal : Official Journal of the Japanese Circulation Society·Hidekazu MaruyamaIwao Yamaguchi
Nov 14, 1997·The Journal of Cell Biology·M A Schwartz
Jul 1, 1992·Baillière's Clinical Haematology·J W Schrader
Feb 1, 1993·The American Journal of Medicine·A SuzukiH Imura
Nov 10, 2005·PLoS Biology·Ganna BilousovaJames DeGregori
Apr 1, 1993·The American Journal of the Medical Sciences·R A Fleischman
Jul 29, 1991·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Metcalf
May 15, 1991·Cancer·D Metcalf
Feb 16, 2019·Growth Factors·Vahid Pazhakh, Graham J Lieschke
Jul 1, 1998·International Reviews of Immunology·W S Alexander
Apr 15, 1994·Annals of the New York Academy of Sciences·W NijhofM Loeffler
Sep 1, 1991·The American Journal of Physiology·J G AdamsM H Steinberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.