Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells.

Archives of Toxicology
Hauke ReimannHenning Hintzsche

Abstract

Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.

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Citations

Sep 20, 2020·International Journal of Cancer. Journal International Du Cancer·Xihan GuoXu Wang
Feb 21, 2021·Seminars in Cell & Developmental Biology·F PellestorV Gatinois
Aug 17, 2021·Journal of Hazardous Materials·Cong-Cong SunLing-Li Zhao

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