Long-term follow-up for the microbiological safety of clinical microencapsulated neonatal porcine islet transplantation.

Xenotransplantation
S MatsumotoCarlos J Wechsler

Abstract

Enrollment in three clinical trials for microencapsulated neonatal porcine islet xenotransplantation to treat unstable type 1 diabetic patients concluded in November 2014. In this study, we report a long-term follow-up assessment of microbiological safety for these trials. Thirty-eight type 1 diabetic patients received microencapsulated neonatal porcine islet transplants. Islets were isolated and prepared from the pancreata of New Zealand (NZ) based designated pathogen-free (DPF) pigs under GMP conditions. Blood samples of thirty-six patients were collected from 5 to 7 years post-first transplant and were tested by real-time PCR for porcine circovirus-1 (PCV1), porcine circovirus-2 (PCV2), porcine lymphotropic herpesvirus 1 (PLHV1), porcine lymphotropic herpesvirus 2 (PLHV2), and porcine cytomegalovirus (PCMV). To detect porcine endogenous retrovirus (PERV), specific real-time PCR and product enhanced reserve transcriptase (PERT) assays were performed. PCV1, PCV2, PLHV1, PLHV2, PCMV, PERV, and reverse transcriptase (RT) activity remained undetected in all tested samples indicating no viral transmission. Except for one patient that died due to complications unrelated to the transplant, there were no significant adverse events. M...Continue Reading

References

Mar 27, 2007·Xenotransplantation·Robert B ElliottChristina Buchanan
Apr 12, 2012·Clinical Microbiology Reviews·Joachim Denner, Ralf R Tönjes
Nov 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anna L HillbergRobert B Elliott
Aug 19, 2014·Transplantation Proceedings·S MatsumotoR B Elliott
Mar 19, 2016·Journal of Diabetes·Shinichi MatsumotoOsamu Sawamoto
Apr 16, 2016·Transplantation·James F MarkmannTimo Otonkoski
Oct 26, 2016·EBioMedicine·Shinichi MatsumotoRobert B Elliott
Nov 5, 2016·Virus Research·Vladimir A MorozovRobert Elliott
Apr 12, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P J Cowan, A J Tector
Apr 21, 2017·Viruses·Joachim Denner, Annette Mankertz
Mar 8, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jay A Fishman
Sep 29, 2018·Xenotransplantation·Caroll B HartlineMark N Prichard
Apr 5, 2020·Journal of Diabetes·Shinichi Matsumoto, Masayuki Shimoda

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Citations

Jan 9, 2021·Journal of Hepato-biliary-pancreatic Sciences·Braulio A Marfil-GarzaTatsuya Kin
Mar 22, 2021·Xenotransplantation·Wayne J HawthorneChristopher Burlak
Apr 24, 2021·World Journal of Diabetes·Masaki NagayaHiroshi Nagashima
May 28, 2021·Biologicals : Journal of the International Association of Biological Standardization·Ken KonoYoji Sato
Aug 31, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Emanuele CozziUNKNOWN for ESOT Workstream 1 of the TLJ (Transplantation Learning Journey) Project

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