Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice

Stem Cells and Development
Panicos ShangarisAnna L David

Abstract

Clinical success of in utero transplantation (IUT) using allogeneic hematopoietic stem cells (HSCs) has been limited to fetuses that lack an immune response to allogeneic cells due to severe immunological defects, and where transplanted genetically normal cells have a proliferative or survival advantage. Amniotic fluid (AF) is an autologous source of stem cells with hematopoietic potential that could be used to treat congenital blood disorders. We compared the ability of congenic and allogeneic mouse AF stem cells (AFSC) to engraft the hematopoietic system of time-mated C57BL/6J mice (E13.5). At 4 and 16 weeks of age, multilineage donor engraftment was higher in congenic versus allogeneic animals. In vitro mixed lymphocyte reaction confirmed an immune response in the allogeneic group with higher CD4 and CD8 cell counts and increased proliferation of stimulated lymphocytes. IUT with congenic cells resulted in 100% of donor animals having chimerism of around 8% and successful hematopoietic long-term engraftment in immune-competent mice when compared with IUT with allogeneic cells. AFSCs may be useful for autologous cell/gene therapy approaches in fetuses diagnosed with congenital hematopoietic disorders.

References

May 29, 2002·Oncogene·Richard A Steinman
Jul 2, 2003·Human Reproduction·Andrea-Romana PrusaMarkus Hengstschläger
Dec 8, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Dario Fauza
Jun 13, 2006·Biochemical and Biophysical Research Communications·Anand S SrivastavaEwa Carrier
Jan 9, 2007·Nature Biotechnology·Paolo De CoppiAnthony Atala
Nov 24, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Eleonor Tiblad, Magnus Westgren
Jan 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Philip J Spence, E Allison Green
May 28, 2008·Transplant Immunology·Margarita M Velásquez-LoperaAndrés Jaramillo
Jun 20, 2008·Stem Cells and Development·Davide GrisafiRosella Tomanin
Feb 18, 2009·Blood·Andrea DitadiIsabelle André-Schmutz
Mar 10, 2011·Current Opinion in Obstetrics & Gynecology·Sheng Wen S ShawPaolo De Coppi
Jan 10, 2012·Annual Review of Immunology·Angelina M Bilate, Juan J Lafaille
Jun 12, 2012·Clinics in Perinatology·Amar NijagalTippi C MacKenzie
Jan 3, 2013·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·James E FisherScott L Nyberg
Feb 26, 2013·American Journal of Reproductive Immunology : AJRI·Trevor D Burt
Dec 18, 2013·Stem Cells Translational Medicine·Cecilia GötherströmJerry K Y Chan
Jan 15, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marta WegorzewskaTippi C MacKenzie
Jun 20, 2014·European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [et Al] = Zeitschrift Für Kinderchirurgie·Stavros P Loukogeorgakis, Alan W Flake
Jan 8, 2015·Frontiers in Pharmacology·Durrgah L RamachandraAnna L David
May 20, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Jeroen B van der NetPaul N Harden
Nov 7, 2015·Best Practice & Research. Clinical Obstetrics & Gynaecology·Stavros P Loukogeorgakis, Paolo De Coppi

❮ Previous
Next ❯

Citations

Apr 5, 2019·Molecular Diagnosis & Therapy·Panayiota PapasavvaCarsten W Lederer
Oct 16, 2019·Stem Cells Translational Medicine·Stuart P Atkinson
May 1, 2020·Current Stem Cell Reports·Martin RodriguezGraҫa Almeida-Porada

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
mechanical dissociation
PCR
FACS

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.