Long-term in vitro analysis of limb cartilage development: involvement of Wnt signaling

Journal of Cellular Biochemistry
Kathleen M DaumerRocky S Tuan

Abstract

Endochondral skeletal development involves the condensation of mesenchymal cells, their differentiation into chondrocytes, followed by chondrocyte maturation, hypertrophy, and matrix mineralization, and replacement by osteoblasts. The Wnt family of secreted proteins have been shown to play important roles in vertebrate limb formation. To examine the role(s) of Wnt members and their transmembrane-spanning receptor(s), Frizzled (fz), we retrovirally misexpressed Wnt-5a, Wnt-7a, chicken frizzled-1 (Chfz-1), and frizzled-7 (Chfz-7) in long-term (21 day) high density, micromass cultures of stage 23/24 chick embryonic limb mesenchyme. This culture system recapitulates in vitro the entire differentiation (days 1-10), growth (days 5-12), and maturation and hypertrophy (from day 12 on) program of cartilage development. Wnt-7a misexpression severely inhibited chondrogenesis from day 7 onward. Wnt-5a misexpression resulted in a poor hypertrophic phenotype by day 14. Chfz-7 misexpression caused a slight delay of chondrocyte maturation based on histology, whereas Chfz-1 misexpression did not affect the chondrogenic phenotype. Misexpression of all Wnt members decreased collagen type X expression and alkaline phosphatase activity at day 21. O...Continue Reading

References

Oct 1, 1977·Developmental Biology·P B AhrensR S Reiter
Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V Hamburger, H L Hamilton
Aug 1, 1992·Current Opinion in Genetics & Development·M E Dickinson, A P McMahon
Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Jun 26, 1992·Cell·R Nusse, H E Varmus
Jun 1, 1986·Developmental Biology·J D San Antonio, R S Tuan
Dec 1, 1994·Experimental Cell Research·S TavellaP Castagnola
Sep 1, 1994·Molecular and Cellular Biology·G T WongA P McMahon
Feb 1, 1997·Developmental Biology·D C SlusarskiR T Moon
May 1, 1997·Developmental Biology·J A Rudnicki, A M Brown
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
Feb 28, 1998·The Biochemical Journal·T C Dale
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
Jan 19, 1999·Current Topics in Developmental Biology·H Dierick, A Bejsovec
Jan 26, 1999·Annals of the New York Academy of Sciences·M GiarréA M Brown
Jun 1, 1999·Methods in Enzymology·P Carninci, Y Hayashizaki
Aug 13, 1999·Development, Growth & Differentiation·Y KawakamiT Nohno
Aug 19, 2000·Methods in Molecular Biology·A M DeLise, R S Tuan
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan
Feb 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Cevik TufanRocky S Tuan
Mar 15, 2003·Developmental Cell·Nicholas S TolwinskiEric Wieschaus
Jul 4, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Isao OishiYasuhiro Minami
Aug 14, 2003·Mechanisms of Development·Cynthia M Coleman, Rocky S Tuan
Sep 6, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Lillian ShumRocky S Tuan
Sep 12, 2003·Developmental Cell·Michael T VeemanRandall T Moon
Jan 28, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Yingzi Yang

❮ Previous
Next ❯

Citations

Sep 6, 2011·The Journal of Biological Chemistry·Noboru TaniguchiMartin Lotz
Jul 13, 2005·DNA and Cell Biology·Karen E YatesRichard G Reish
Sep 2, 2008·Tissue Engineering. Part a·Catherine K Kuo, Rocky S Tuan
Jul 31, 2008·PloS One·Gidon OfekKyriacos A Athanasiou
May 21, 2013·The Journal of Hand Surgery·Mohammad M Al-Qattan, Scott H Kozin
Apr 1, 2006·Journal of Cellular Physiology·Yu-Feng DongHicham Drissi
Aug 19, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jae-Beom KimJill A Helms
Aug 5, 2006·Journal of Cellular Biochemistry·Eric P PourmandAdele L Boskey
Feb 24, 2011·American Journal of Medical Genetics. Part a·Wafaa EyaidMohammed Al Balwi
Jun 21, 2012·Cell Biochemistry and Function·Katherine Ann StainesColin Farquharson
Aug 25, 2009·The Journal of Hand Surgery·Mohammad M Al-QattanScott H Kozin
Aug 7, 2008·BMB Reports·Jang-Soo ChunMeeyoung Park
Apr 18, 2012·The Biochemical Journal·Seung-Won ChoiDae-Won Kim
Dec 7, 2010·Mechanisms of Development·Dmitry NurminskyMaria Nurminskaya
Mar 12, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Sara Hosseini-FarahabadiJoy M Richman
Jun 14, 2005·Developmental Biology·Luis de la Fuente, Jill A Helms
Sep 27, 2005·The International Journal of Biochemistry & Cell Biology·Eun-Jung JinShin-Sung Kang
Aug 17, 2010·The Journal of Hand Surgery, European Volume·M M Al-Qattan
Mar 14, 2009·The Journal of Hand Surgery, European Volume·M M Al-QattanS Al-Turki
Sep 17, 2017·Human Cell·Noboru TaniguchiMartin Lotz
Sep 7, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Maria A Mello, Rocky S Tuan
Sep 30, 2008·Nature Clinical Practice. Rheumatology·Maripat Corr
Sep 1, 2006·Journal of the American Society of Nephrology : JASN·Chun-Liang LinFeng-Sheng Wang
Apr 12, 2019·Environmental Science and Pollution Research International·Khalid MehmoodJiakui Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.