Long-term induction of haem oxygenase-1 (HSP-32) in astrocytes and microglia following transient focal brain ischaemia in the rat

The European Journal of Neuroscience
J KoistinahoJ T Laitinen

Abstract

Haem oxygenase-1 (HO-1) is a stress protein and a rate-limiting enzyme in haem degradation, generating ferrous iron, carbon monoxide and bile pigments. HO-1 has been suggested to be protective against oxidative stress. In the normal rodent brain the enzyme is localized in selected neuron populations, but heat shock, glutathione depletion in vivo and oxidative stress in vitro induce HO-1 predominantly in glial cells. We studied HO-1 expression in the brain following transient occlusion of the middle cerebral artery, and found increased mRNA levels in the ischaemic region from 4 h to 7 days after 90 min of ischaemia. The mRNA levels peaked at 12 h, and were localized perifocally. HO-1-immunoreactive astrocytes and microglial cells were seen in the perifocal area, in the ipsilateral and occasionally in the contralateral hippocampus. Some perifocal neurons were also HO-1-immunoreactive. In the infarcted area HO-1-positive microglia/macrophages were detected in double-labelling experiments. A microassay measuring the conversion of [14C]haem to [14C]bilirubin showed a two-fold increase in haem oxygenase activity in the infarcted core. These observations show a long-term induction of HO-1 protein and its activity following ischaemia-r...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H KinouchiP H Chan
Oct 1, 1991·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·T W WuD Carey
May 1, 1991·Trends in Pharmacological Sciences·G S MarksB E McLaughlin
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R S EisensteinH N Munro
Dec 1, 1989·Trends in Biochemical Sciences·G PadmanabanP N Rangarajan
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S ShibaharaT Yoshida
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·N G AbrahamA Kappas
Jan 15, 1993·Science·A VermaS H Snyder
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R JaffreyS H Snyder
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·R K KuttyD T Organisciak
Oct 10, 1994·Neuroscience Letters·W PaschenJ Schmitt
Jan 1, 1995·Journal of Neurosurgical Anesthesiology·B K SiesjöT Kristián
Dec 1, 1993·Brain Research. Molecular Brain Research·T FuruyamaH Takagi
Jun 1, 1993·Experimental Neurology·Y Amit, T Brenner
Oct 4, 1993·FEBS Letters·J Neuzil, R Stocker
Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H KinouchiP H Chan
Apr 1, 1993·Journal of Neurochemistry·J F Ewing, M D Maines

❮ Previous
Next ❯

Citations

Apr 13, 1999·Trends in Neurosciences·F R SharpR A Swanson
Dec 14, 2002·Brain Research. Molecular Brain Research·Rainald Schmidt-KastnerMyron D Ginsberg
Feb 13, 2001·The European Journal of Neuroscience·K ShibukiH Wakatsuki
Nov 20, 2002·Journal of Neurochemistry·Vemuganti L Raghavendra RaoRobert J Dempsey
Aug 24, 2005·Antioxidants & Redox Signaling·Ralf Dringen
May 9, 2006·Antioxidants & Redox Signaling·Giovanni ScapagniniVittorio Calabrese
Feb 5, 2002·Annual Review of Physiology·Gabriele V Ronnett, Cheil Moon
Dec 17, 2009·Redox Report : Communications in Free Radical Research·Stephen R RobinsonGlenda M Bishop
Oct 12, 2000·Journal of Neuroimmunology·H J Schluesener, K Seid
Sep 23, 2011·Journal of Neuropathology and Experimental Neurology·Starlee LivelyLyanne C Schlichter
Feb 16, 2007·Neuroscience Letters·Hyun Joo ShinGu Seob Roh
Aug 12, 2008·Journal of Neurochemistry·Helena L A VieiraPaula M Alves
Jul 29, 2004·Epilepsy Research·Xiao-Yuan Lian, Janet L Stringer
Jul 11, 2006·Neuroscience·A S AhmadS Doré
Jul 25, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·F R SharpD E Millhorn
Jun 8, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·John P MacManusJacqueline Webster
Jun 11, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kenneth R WagnerJoseph F Clark
Apr 5, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Tiago J T P MoreiraSture Liljequist
Sep 8, 2017·International Journal of Molecular Sciences·Laura Terraneo, Michele Samaja
Jan 8, 1999·Stroke; a Journal of Cerebral Circulation·J KoistinahoP H Chan
Mar 8, 2008·Stroke; a Journal of Cerebral Circulation·Carles JusticiaMathias Hoehn
Mar 10, 2007·Journal of Neuroscience Research·Myung-Soon YangEunhye Joe
Sep 28, 2005·Neurochemical Research·Stéphane WoerlyAraceli Espinosa-Jeffrey
Feb 21, 2002·Molecular Pharmacology·G ScapagniniR Motterlini

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