Long-term influence of bone marrow-derived mesenchymal stem cells on liver ischemia-reperfusion injury in a rat model

Annals of Transplantation : Quarterly of the Polish Transplantation Society
Maciej NowackiTomasz Drewa

Abstract

The aim of this study was to evaluate the long-term usefulness of intraportal injection of the bone marrow-derived mesenchymal stem cells (BM-MSCs) in limitation of experimentally induced ischemia-reperfusion injury (IRI) in a rat model. Twenty Wistar rats were divided into 3 groups: donor group (n=5), study group (n=10), and control group (n=5). IRI was performed using a modified hanging-weight system after left portal triad occlusion in study group animals. Isolated autologous BM-MSCs were labeled with fluorochrome PKH-26 then intraportally injected into the rats in the study group. Control group animals were intraportally injected with 1 ml of PBS. Follow-up was 3 months, after which animals were sacrificed for histopathological examination. Migration of BM-MSCs into different organs was examined. H&E staining of liver tissue sections from "time zero" biopsies did not show many irregularities in structural or histological construction compared to liver sections from the control group. However, a small amount of centrilobular hepatocyte necrosis and coagulative necrosis with neutrophil infiltration areas was observed in liver sections of the study group. The migration assay of BM-MSCs labeled with PKH-26 showed the highest po...Continue Reading

Citations

Feb 24, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xian-Kui CaoBao-Lin Liu
Jul 2, 2016·The Journal of Surgical Research·Askhat IsbambetovSusumu Eguchi
Apr 8, 2017·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Badri Shrestha, John Haylor
Dec 19, 2017·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Badri Man Shrestha, John Haylor
Apr 14, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Hiroaki HagaTushar Patel
Dec 26, 2018·Journal of Biomedical Science·Steven Ting-Yu ChuoCharles Pin-Kuang Lai
Jul 29, 2021·JTCVS Techniques·Asvin M Ganapathi, Nahush A Mokadam

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