Long-term measurement of renal cortical and medullary tissue oxygenation and perfusion in unanesthetized sheep

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Paolo CalzavaccaC N May

Abstract

The role of renal cortical and medullary hypoxia in the development of acute kidney injury is controversial, partly due to a lack of techniques for the long-term measurement of intrarenal oxygenation and perfusion in conscious animals. We have, therefore, developed a methodology to chronically implant combination probes to chronically measure renal cortical and medullary tissue perfusion and oxygen tension (tPO2) in conscious sheep and evaluated their responsiveness and reliability. A transit-time flow probe and a vascular occluder were surgically implanted on the left renal artery. At the same operation, dual fiber-optic probes, comprising a fluorescence optode to measure tPO2 and a laser-Doppler probe to assess tissue perfusion, were inserted into the renal cortex and medulla. In recovered conscious sheep (n = 8) breathing room air, mean 24-h cortical and medullary tPO2 were similar (31.4 ± 0.6 and 29.7 ± 0.7 mmHg, respectively). In the renal cortex and medulla, a 20% reduction in renal blood flow (RBF) decreased perfusion (14.6 ± 8.6 and 41.2 ± 8.5%, respectively) and oxygenation (48.1 ± 8.5 and 72.4 ± 8.5%, respectively), with greater decreases during a 50% reduction in RBF. At autopsy, minimal fibrosis was observed around ...Continue Reading

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Citations

Jun 10, 2016·Mathematical Medicine and Biology : a Journal of the IMA·Ioannis SgouralisAnita T Layton
Nov 22, 2017·Acta Physiologica·C P C OwR G Evans
Jul 8, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ioannis SgouralisRoger G Evans
Oct 18, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yugeesh R LankadevaClive N May
Dec 5, 2018·Artificial Organs·Thomas MinorAndreas Paul
Feb 21, 2019·American Journal of Respiratory and Critical Care Medicine·Naoya IguchiClive N May
Dec 12, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roger G EvansYugeesh R Lankadeva
Apr 22, 2017·Frontiers in Physiology·Tonja W EmansC T Paul Krediet
Sep 12, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paolo CalzavaccaClive N May
May 30, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Naoya IguchiClive N May
Feb 26, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roger G Evans
Aug 29, 2019·American Journal of Physiology. Renal Physiology·Md Mahbub UllahRoger G Evans
Mar 5, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jessica R AbrahamsonGregory M T Hare
Oct 28, 2020·Clinical and Translational Science·Thomas Minor, Charlotte von Horn
Aug 31, 2021·American Journal of Physiology. Renal Physiology·Chang-Joon LeeDavid W Smith

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