Long-term moderate ethanol consumption restores insulin sensitivity in high-fat-fed rats by increasing SLC2A4 (GLUT4) in the adipose tissue by AMP-activated protein kinase activation

The Journal of Endocrinology
Li FengJia-jun Zhao

Abstract

The sole effect of either saturated fatty acid or moderate ethanol consumption on SLC2A4 (GLUT4) expression is widely reported but the combined effects of them remain obscure. Here, we observed their combined effects on SLC2A4 expression, explored the underlying mechanism mediated by AMP-activated protein kinase alpha (PRKAA2) and myocyte enhancer factor 2 (MEF2) both in vivo and in vitro. In the in vivo experiments, 36 male Wistar rats, divided into three groups, were fed with normal diet, high-fat (HF) diet, or HF diet plus ethanol for 22 weeks. We measured the expressions of total-PRKAA2 (T-PRKAA2), phosphorylated-PRKAA2 (pPRKAA2, activated form of PRKAA2), MEF2, and SLC2A4 in epididymal adipose tissues. In the in vitro experiments, primary adipocytes, isolated from normal Wistar rats, were incubated in the presence or absence of palmitate, ethanol, and compound C (an PRKAA2 inhibitor) for 1 h. Thereafter, T-PRKAA2, pPRKAA2, MEF2, and SLC2A4 expressions were measured. We found that both HF diet and in vitro exposition to palmitate impaired SLC2A4 expression in rat adipocytes with a parallel reduction in PRKAA2 activation and MEF2 expression. This impairment was reversed by ethanol administration. We further demonstrated that...Continue Reading

Citations

Mar 6, 2010·Cancer Letters·Jina HongNomelí P Núñez
Mar 4, 2015·Journal of Gastroenterology·Hirokazu TakahashiYuichiro Eguchi
Jan 1, 2015·Journal of Drug and Alcohol Research·Kamlesh K BhopaleG A Shakeel Ansari
Feb 18, 2017·Biomolecules·Jennifer L Steiner, Charles H Lang
Sep 21, 2017·Nature Reviews. Gastroenterology & Hepatology·Richard ParkerBin Gao

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