Long-term monitoring reveals stable and remarkably similar microbial communities in parallel full-scale biogas reactors digesting energy crops

FEMS Microbiology Ecology
Rico LucasSabine Kleinsteuber

Abstract

Biogas is an important renewable energy carrier. It is a product of stepwise anaerobic degradation of organic materials by highly diverse microbial communities forming complex interlinking metabolic networks. Knowledge about the microbial background of long-term stable process performance in full-scale reactors is crucial for rationally improving the efficiency and reliability of biogas plants. To generate such knowledge, in the present study three parallel mesophilic full-scale reactors fed exclusively with energy crops were sampled weekly over one year. Physicochemical process parameters were determined and the microbial communities were analysed by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting and 454-amplicon sequencing. For investigating the methanogenic community, a high-resolution T-RFLP approach based on the mcrA gene was developed by selecting restriction enzymes with improved taxonomic resolution compared to previous studies. Interestingly, no Methanosarcina-related generalists, but rather specialized hydrogenotrophic and acetoclastic methanogenic taxa were detected. In general, the microbial communities in the non-connected reactors were remarkably stable and highly similar indicating that...Continue Reading

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Citations

Sep 29, 2015·Applied Microbiology and Biotechnology·Heike SträuberSabine Kleinsteuber
Dec 10, 2015·Frontiers in Microbiology·Hyun-Seob SongStephen R Lindemann
Mar 21, 2016·Applied Microbiology and Biotechnology·Tobias KernMichael Rother
Dec 27, 2016·Frontiers in Microbiology·Babett WintscheSabine Kleinsteuber
Feb 15, 2018·Microorganisms·Emine Gozde OzbayramSabine Kleinsteuber
Sep 12, 2019·Bioprocess and Biosystems Engineering·Cecilia CallejasClaudia Etchebehere
Jan 6, 2018·AMB Express·Franziska EnzmannDirk Holtmann
Nov 21, 2017·Applied Microbiology and Biotechnology·Emine Gozde OzbayramOrhan Ince
Dec 5, 2019·Applied Microbiology and Biotechnology·Emine Gozde OzbayramMarcell Nikolausz
Mar 28, 2019·Applied Microbiology and Biotechnology·Ayrat M ZiganshinSabine Kleinsteuber
Jun 5, 2019·Applied Microbiology and Biotechnology·Masanori FujimotoPatrick J McNamara
Oct 20, 2018·Applied Microbiology and Biotechnology·Zuopeng LvMarcell Nikolausz
Sep 27, 2019·Frontiers in Microbiology·Martin A FischerRuth A Schmitz
Feb 9, 2021·Frontiers in Microbiology·Patricia Bovio-WinklerClaudia Etchebehere
Aug 3, 2021·The Science of the Total Environment·Doorgha RagooburGeeta Devi Somaroo
Feb 21, 2018·Environmental Science & Technology·Stefanie KahlThorsten Reemtsma
Apr 11, 2019·Environmental Science & Technology·Raquel LiébanaBritt-Marie Wilén

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