Long-term nutrient starvation of continuously cultured (glucose-limited) Selenomonas ruminantium.

Journal of Bacteriology
R W Mink, R B Hespell

Abstract

Selenomonas ruminantium, a strictly anaerobic ruminal bacterium, was grown at various dilution rates (D = 0.05, 0.25, and 0.35 h-1) under glucose-limited continuous culture conditions. Suspensions of washed cells prepared anaerobically in mineral buffer were subjected to nutrient starvation (24 to 36 h; 39 degrees C; N2 atmosphere). Regardless of growth rate, viability declined logarithmically, and within about 2.5 h, about 50% of the populations were nonviable. After 24 h of starvation, the numbers of viable cells appeared to be inversely related to growth rate, the highest levels occurring with the slowest grown population. Cell dry weight, carbohydrate, protein, ribonucleic acid (RNA), and deoxyribonucleic acid declined logarithmically during starvation, and the decline rates of each were generally greater with cells grown at higher D values. Both cellular carbohydrate and RNA declined substantially during the first 12 h of starvation. Most of the cellular RNA that disappeared was found in the suspending buffer as low-molecular-weight, orcinol-positive materials. During growth, S. ruminantium made a variety of fermentation acids from glucose, but during starvation, acetate was the only acid made from catabolism of cellular m...Continue Reading

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Aug 1, 1996·Journal of Dairy Science·J E Wells, J B Russell
Jan 19, 1999·Journal of Dairy Science·J DijkstraD R Davies
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Jan 1, 1991·Applied and Environmental Microbiology·H J Strobel, J B Russell
Apr 1, 1997·Applied and Environmental Microbiology·J LouH J Strobel

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