Abstract
Time-lapse in vivo microscopy studies of cellular morphology and physiology are crucial toward understanding brain function but have been infeasible in the fruit fly, a key model species. Here we use laser microsurgery to create a chronic fly preparation for repeated imaging of neural architecture and dynamics for up to 50 days. In fly mushroom body neurons, we track axonal boutons for 10 days and record odor-evoked calcium transients over 7 weeks. Further, by using voltage imaging to resolve individual action potentials, we monitor spiking plasticity in dopamine neurons of flies undergoing mechanical stress. After 24 h of stress, PPL1-α'3 but not PPL1-α'2α2 dopamine neurons have elevated spike rates. Overall, our chronic preparation is compatible with a broad range of optical techniques and enables longitudinal studies of many biological questions that could not be addressed before in live flies.
References
Dec 20, 2002·Nature·Joshua T TrachtenbergKarel Svoboda
Jun 9, 2006·Nature·Jena L PitmanRavi Allada
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Jun 27, 2008·Journal of Neuroscience Methods·Peter B AllenDaniel T Chiu
Oct 20, 2009·Cell·Adam Claridge-ChangGero Miesenböck
Jun 8, 2010·Nature Methods·Johannes D SeeligVivek Jayaraman
Aug 11, 2010·Genetics·Barret D PfeifferGerald M Rubin
Mar 1, 2012·Experimental Neurology·Gayane Aramuni, Oliver Griesbeck
Aug 9, 2012·Development·Anna KaufmannJan Huisken
Jan 22, 2013·Biophysical Journal·Brian A WiltMark J Schnitzer
Jul 23, 2013·Nature·Tsai-Wen ChenDouglas S Kim
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Supriyo SinhaMark J Schnitzer
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ming-Chin WuAnn-Shyn Chiang
Dec 24, 2014·ELife·Yoshinori AsoGerald M Rubin
May 26, 2015·Nature Methods·Joan SavallMark J Schnitzer
Nov 21, 2015·Science·Yiyang GongMark J Schnitzer
Dec 22, 2015·Cell·Raphael CohnVanessa Ruta
Feb 27, 2016·Science·Xitong LiangPaul H Taghert
May 18, 2016·Nature Methods·Dhruv GroverRalph J Greenspan
Jul 22, 2016·ELife·Yoshinori Aso, Gerald M Rubin
Citations
Nov 14, 2018·Nature Methods·Madhuvanthi KannanVincent A Pieribone
Dec 27, 2019·Journal of Neurogenetics·Sophie Aimon, Ilona C Grunwald Kadow
Feb 2, 2020·Brain Structure & Function·Christian R LeeDavid J Margolis
Jul 12, 2020·IScience·Lorena de Mena, Diego E Rincon-Limas
Jan 10, 2021·Neuroscience and Biobehavioral Reviews·Jiří Dvořáček, Dalibor Kodrík
Mar 7, 2021·Sensors·Pikam PunDal Hyung Kim
Apr 9, 2021·Neuron·Zhe Sage Chen, Bijan Pesaran
Aug 28, 2021·Cells·Nelly RedolfiDiana Pendin
Oct 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jia JiaHongtao Qin
Sep 11, 2020··Nilay YapiciAaron T. Mok