Long-term preservation of islets of Langerhans in hydrophilic macrobeads

Transplantation
K JainA L Rubin

Abstract

Several obstacles have hindered the successful transplantation of islets of Langerhans to human patients in efforts to cure type I diabetes mellitus. One problem is the necessity for short- and long-term storage of islets after isolation and before transplantation. Current long-term storage methods, such as incubation in a physiological medium and cryopreservation, are suboptimal, resulting in significant loss of viable islet mass or function. Better storage methods are needed. In this study we examined the long-term storage of rat islets in macrobeads composed of agarose and collagen. Islets isolated from Wistar-Furth rats were placed into macrobeads (1000 islets/macrobead) and maintained in culture for periods of up to 189 days at 37 degrees C. Insulin released from the cultured macrobeads remained constant for periods of at least 154 days. In one group, insulin release was 1050 mU/24 hr/4 beads on day 3 and 1040 mU/24 hr/4 beads on day 154. In another group, insuling release was 1305 Xenotransplantation of Wistar Furth islet macrobeads, stored for 10 to 112 days at 37 degrees C, degrees C into 42 B6AF/1 mice with streptozotocin-induced diabetes resulted in a return to euglycemia in the recipients within 24 hr. Thereafter, eu...Continue Reading

References

Jul 1, 1992·Journal of Biomedical Materials Research·H IwataT Akutsu
Oct 1, 1985·Transplantation·M GotohA P Monaco
Oct 1, 1987·Cryobiology·N H JutteG H Zeilmaker
Jul 1, 1994·Cell Transplantation·G S Korbutt, D G Pipeleers

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Citations

Jan 18, 2006·Tissue Engineering·Ali KhademhosseiniMichael V Sefton
Nov 26, 2013·Advanced Drug Delivery Reviews·Paul de VosMarijke M Faas
Aug 1, 2015·Journal of Biomaterials Science. Polymer Edition·L DraghiM C Tanzi
Jun 5, 2016·Biochemical and Biophysical Research Communications·Pradeep R DumpalaLawrence S Gazda

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