Long-term recovery after bone marrow stromal cell treatment of traumatic brain injury in rats

Journal of Neurosurgery
Asim MahmoodMichael Chopp

Abstract

This study was designed to follow the effects of bone marrow stromal cell (BMSC) administration in rats after traumatic brain injury (TBI) for a 3-month period. Forty adult female Wistar rats were injured by a controlled cortical impact and, 1 week later, were injected intravenously with one of three different doses of BMSCs (2 x 10(6), 4 x 10(6), or 8 x 10(6) cells per animal) obtained in male rats. Control rats received phosphate-buffered saline (PBS). Neurological function in these rats was studied using a neurological severity scale (NSS). The rats were killed 3 months after injury, and immunohistochemical stains were applied to brain samples to study the distribution of the BMSCs. Additional brain samples were analyzed by quantitative enzyme-linked immunosorbent assays to measure the expression of the growth factors brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Three months after injury, BMSCs were present in the injured brain and their number was significantly greater in animals that received 4 x 10(6) or 8 x 10(6) BMSCs than in animals that received 2 x 10(6) BMSCs. The cells were primarily distributed around the lesion boundary zone. Functional outcome was significantly better in rats that rece...Continue Reading

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Citations

Jun 30, 2009·Neuroradiology·Johan LundbergStaffan Holmin
Aug 15, 2009·The Journal of Trauma·Peter A WalkerCharles S Cox
Sep 29, 2007·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Runjiang QuMichael Chopp
Jan 28, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Katsuhiko MaruichiYoshinobu Iwasaki
Jan 10, 2009·Disease Models & Mechanisms·Peter A WalkerCharles S Cox
May 30, 2013·PloS One·Shiyang WangQuan Jiang
Mar 24, 2009·Journal of Neurosurgery·Matthew T HartingCharles S Cox
Mar 18, 2008·Neurosurgical Focus·Matthew T HartingCharles S Cox
Aug 1, 2009·Stem Cell Reviews and Reports·Peter A WalkerCharles S Cox
Jun 8, 2010·Stem Cell Reviews and Reports·Chahrazad MoubarikPascale Pisano
Jan 19, 2013·Nature Reviews. Neuroscience·Ye XiongMichael Chopp
Mar 3, 2009·Expert Opinion on Emerging Drugs·Ye XiongMichael Chopp
Aug 7, 2010·Comptes rendus biologies·Karine CharrièreDominique Fellmann
Mar 17, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Xue-Yuan Li, Dong-Fu Feng
May 12, 2009·Neuroscience Letters·Yi Li, Michael Chopp
Nov 13, 2007·Brain Research·Aki ShintaniToru Itakura
Jul 6, 2007·Journal of the Neurological Sciences·Michael ChoppJing Zhang
Feb 7, 2016·World Neurosurgery·Melek Tunc AtaSebahat Turgut
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Apr 2, 2015·Molecular and Cellular Neurosciences·Erica D BruceDeborah M Little
Jun 25, 2016·Stem Cells International·Markus Huber-LangRolf E Brenner

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Methods Mentioned

BETA
flow cytometry
ELISA
light microscopy
fluorescence microscopy
ELISAs
protein assay

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Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.