Long-term repopulating ability of telomerase-deficient murine hematopoietic stem cells.

Blood
E SamperMiguel Aracil

Abstract

Telomere length must be tightly regulated in highly proliferative tissues, such as the lymphohematopoietic system. Under steady-state conditions, the levels and functionality of hematopoietic-committed or multipotent progenitors were not affected in late-generation telomerase-deficient mice (mTerc(-/-)) with critically short telomeres. Evaluation of self-renewal potential of mTerc(-/-) day-12 spleen colony-forming units demonstrated no alteration as compared with wildtype progenitors. However, the replating ability of mTerc(-/-) granulocyte-macrophage CFUs (CFU-GMs) was greatly reduced as compared with wildtype CFU-GMs, indicating a diminished capacity of late-generation mTerc(-/-) committed progenitors when forced to proliferate. Long-term bone marrow cultures of mTerc(-/-) bone marrow (BM) cells show a reduction in proliferative capacity; this defect can be mainly attributed to the hematopoietic, not to the stromal, mTerc(-/-) cells. In serial and competitive transplantations, mTerc(-/-) BM stem cells show reduced long-term repopulating capacity, concomitant with an increase in genetic instability compared with wildtype cells. Nevertheless, in competitive transplantations late-generation mTerc(-/-) precursors can occasionally...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R C AllsoppC B Harley
Sep 1, 1992·International Journal of Cell Cloning·G J Spangrude
Jan 1, 1992·Annual Review of Biochemistry·E H Blackburn
Apr 18, 1991·Nature·E H Blackburn
Aug 11, 1991·Nucleic Acids Research·P W LairdA Berns
May 31, 1990·Nature·C B HarleyC W Greider
Dec 1, 1995·American Journal of Hematology·D A VolpeE C Kohn
Dec 8, 1995·Science·V A Zakian
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·C Härle-Bachor, P Boukamp
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·J M ZijlmansP M Lansdorp
Nov 14, 1997·Genes & Development·W E WrightJ W Shay
Mar 7, 1998·Bone Marrow Transplantation·M AkiyamaH Mizoguchi
May 30, 1998·Genes & Development·C I Nugent, V Lundblad
May 26, 1999·Experimental Hematology·G KellerS Robertson
Feb 2, 2000·The EMBO Journal·E HerreraM A Blasco

❮ Previous
Next ❯

Citations

Jul 9, 2011·Biogerontology·I BellantuonoW N Keith
Mar 9, 2011·International Journal of Hematology·Kazuhito Naka, Atsushi Hirao
Apr 28, 2011·International Journal of Hematology·Jichun Chen
May 14, 2011·International Journal of Hematology·Lijian ShaoDaohong Zhou
Jan 22, 2004·Archives of Medical Research·Matthew J Greenwood, Peter M Lansdorp
Feb 26, 2004·Experimental Cell Research·Lin LiuDavid L Keefe
May 22, 2003·Cancer Letters·María A Blasco
Jun 18, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olga S KustikovaChristopher Baum
Sep 2, 2005·Nature Reviews. Genetics·Maria A Blasco
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Bai-Wei GuPhilip J Mason
Oct 13, 2006·Antioxidants & Redox Signaling·Jan KajsturaAnnarosa Leri
Jul 17, 2008·Antioxidants & Redox Signaling·Kazuhito NakaAtsushi Hirao
Dec 3, 2008·Cardiovascular Research·Liza S M WongPim van der Harst
Dec 28, 2007·Nucleic Acids Research·Jonathan Kenyon, Stanton L Gerson
Nov 23, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Hannes M FindeisenDennis Bruemmer
Sep 18, 2008·American Journal of Respiratory and Critical Care Medicine·Victor J Thannickal, James E Loyd
Jan 15, 2004·The Journal of Clinical Investigation·In-Kyung ParkMichael F Clarke
Feb 2, 2008·Blood·Rodrigo T Calado, Neal S Young
Oct 7, 2005·International Journal of Hematology·Jichun Chen
Mar 15, 2006·International Journal of Hematology·Fumio AraiToshio Suda
Nov 19, 2009·Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·Atsushi Hirao
May 15, 2010·Future Oncology·Michelle F MaritzKaren L MacKenzie
Oct 16, 2013·Antioxidants & Redox Signaling·Lijian ShaoDaohong Zhou
Nov 21, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·María A Blasco
Dec 20, 2013·Stem Cells and Development·Lien-Cheng HsiaoCarolyn A Carr
Feb 25, 2011·Nature Reviews. Cancer·Paula Martínez, María A Blasco
Sep 19, 2007·Nature Chemical Biology·Maria A Blasco
Dec 29, 2006·Nature Genetics·Jessica F Bell, Norman E Sharpless
Feb 28, 2007·Leukemia & Lymphoma·Claudia Orelio, Elaine Dzierzak
Jul 10, 2009·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Albert Stuart Reece
Sep 18, 2012·European Journal of Cell Biology·Gayatri RamakrishnaSapna Singh
Feb 24, 2010·Current Opinion in Genetics & Development·Rosa M Marión, Maria A Blasco
Sep 24, 2008·Seminars in Hematology·Roi GazitDerrick J Rossi
Nov 22, 2007·Biochimie·Zhenyu JuLenhard Rudolph
Feb 4, 2006·Stem Cells·Leonie M Kamminga, Gerald de Haan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.