Abstract
Reduced quantity and quality of stem cells in aged individuals hinders cardiac repair and regeneration after injury. We used young bone marrow (BM) stem cell antigen 1 (Sca-1) cells to reconstitute aged BM and rejuvenate the aged heart, and examined the underlying molecular mechanisms. BM Sca-1+ or Sca-1- cells from young (2-3 months) or aged (18-19 months) GFP transgenic mice were transplanted into lethally irradiated aged mice to generate 4 groups of chimeras: young Sca-1+ , young Sca-1- , old Sca-1+ , and old Sca-1- . Four months later, expression of rejuvenation-related genes (Bmi1, Cbx8, PNUTS, Sirt1, Sirt2, Sirt6) and proteins (CDK2, CDK4) was increased along with telomerase activity and telomerase-related protein (DNA-PKcs, TRF-2) expression, whereas expression of senescence-related genes (p16INK4a , P19ARF , p27Kip1 ) and proteins (p16INK4a , p27Kip1 ) was decreased in Sca-1+ chimeric hearts, especially in the young group. Host cardiac endothelial cells (GFP- CD31+ ) but not cardiomyocytes were the primary cell type rejuvenated by young Sca-1+ cells as shown by improved proliferation, migration, and tubular formation abilities. C-X-C chemokine CXCL12 was the factor most highly expressed in homed donor BM (GFP+ ) cells i...Continue Reading
References
May 12, 1998·Circulation Research·W HuangM J Osborne-Pellegrin
Apr 2, 1999·Cell·A BrunetM E Greenberg
Nov 9, 2000·The Journal of Experimental Medicine·K SudoH Nakauchi
Jan 8, 2003·Circulation·Edward G Lakatta, Daniel Levy
Mar 6, 2004·Circulation Research·Maurizio C Capogrossi
Apr 14, 2005·Blood·Ying LiangStephen J Szilvassy
Jul 25, 2007·Circulation·Xiaofeng HuGregg Rokosh
Apr 17, 2008·Blood·Rebecca H ChoChrista E Muller-Sieburg
Apr 19, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Xianjie ZhangJohn W Harmon
Jun 29, 2011·Biochimica Et Biophysica Acta·Guri TzivionGopalakrishnan Ramakrishnan
Aug 2, 2011·Trends in Immunology·Irina B MazoUlrich H von Andrian
Aug 23, 2011·Atherosclerosis·Qiyuan XuHong Yu
Jan 20, 2012·Nature·Christopher J Lord, Alan Ashworth
Feb 22, 2012·Circulation Research·Gian Paolo FadiniStefanie Dimmeler
Mar 1, 2012·Frontiers in Physiology·K WilliamsonM Y Alexander
Apr 18, 2012·European Heart Journal·Shu-Hong LiRen-Ke Li
Jun 7, 2014·Aging·Shufeng ChenJinying Zhao
May 23, 2015·Circulation Research·Opher S KornfeldDaria Mochly-Rosen
Jun 25, 2015·Stem Cells International·Shan GengYa-Ping Wang
Jul 28, 2015·Journal of Molecular and Cellular Cardiology·Nikunj R Shah, Michael Mahmoudi
Sep 24, 2015·The Journal of Physiology·Sarah CostantinoFrancesco Cosentino
Nov 7, 2015·European Heart Journal·Giovanni G CamiciThomas F Lüscher
Jan 20, 2016·Science·Margaret A Goodell, Thomas A Rando
Jul 11, 2016·The Canadian Journal of Cardiology·Kaitlyn M Keller, Susan E Howlett
Feb 1, 2017·Scientific Reports·Shu-Hong LiRen-Ke Li
Citations
May 12, 2020·Journal of Cellular Physiology·Wenzheng XiaShiting Li
Feb 7, 2020·Aging·Faisal J Alibhai, Ren-Ke Li
Aug 23, 2020·Cells·Stephanie W TobinRen-Ke Li
Oct 10, 2020·World Journal of Stem Cells·Qing-Shu MengXiao-Ting Liang
Nov 21, 2020·Aging·Faisal J Alibhai, Ren-Ke Li
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Jun DongJiao Li
Jun 8, 2021·Frontiers in Cardiovascular Medicine·Jielei GuShiming Liu
May 9, 2021·Cardiovascular Research·Maggie S ChenJessica C Garbern
Jul 22, 2021·Seminars in Cell & Developmental Biology·Yi WangDa-Zhi Wang
Sep 29, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Arushi TanejaNagalingam Ravi Sundaresan
Oct 7, 2021·Aging Cell·Tina B MarvastiRen-Ke Li