Long-term reproducibility of in vivo measures of specific binding of radioligands in rat brain

Nuclear Medicine and Biology
Michael R Kilbourn

Abstract

The long-term reproducibility of measures of in vivo specific binding of radiolabeled forms of (+)-alpha-dihydrotetrabenazine (DTBZ) and d-threo-methylphenidate (MPH) in rat brain was examined. All studies were done using a consistent bolus plus infusion protocol and calculation of equilibrium distribution volume ratios (DVR). Over a period of eight years striatal DVR values for DTBZ binding to the vesicular monoamine transporter 2 (VMAT2) in young adult (8-10 wks old) rats showed very good reproducibility (3.62+/-0.33, N=35). Equivalent values were obtained using either tritiated or carbon-11 labeled DTBZ, and were irrespective of sex of animals. Older animals (78 wks old) showed losses (-45%) of specific binding. Striatal binding of MPH to the dopamine transporter (DAT) showed a similar reproducibility over a five year period (DVR=2.17+/-0.39, N=52), again irrespective of radionuclide or sex. These studies demonstrate that use of a consistent in vivo technique can provide reliable measures of specific binding of radioligands to high affinity sites in the rat brain.

References

Nov 25, 2000·Nuclear Medicine and Biology·R E Carson
Feb 15, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M B SkaddanP S Sherman
Oct 14, 2003·Experimental Neurology·V M GoettlM Hadjiconstantinou

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Citations

Dec 1, 2006·Neuropsychiatric Disease and Treatment·Feng LiuRaul R Silva
Jan 26, 2011·European Journal of Pharmacology·Michael R Kilbourn, Edward F Domino
Feb 4, 2010·Nuclear Medicine and Biology·Michael R KilbournKirk A Frey
Mar 27, 2007·Nuclear Medicine and Biology·Michael R KilbournHank F Kung
Aug 13, 2011·Nuclear Medicine and Biology·Laura RavasiDale O Kiesewetter
Mar 17, 2018·Neurology India·R SheelakumariRamshekhar Menon

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