Long-term safety from transmission of porcine endogenous retrovirus after pig-to-non-human primate corneal transplantation

Xenotransplantation
Hyuk Jin ChoiEung Soo Hwang

Abstract

The risk of xenozoonosis mainly by porcine endogenous retrovirus (PERV) has been considered as one of the main hurdles in xenotransplantation and therefore should be elucidated prior to the clinical use of porcine corneal grafts. Accordingly, an investigation was performed to analyze the infectivity of PERVs from porcine keratocytes to human cells, and the long-term risk of transmission of PERVs was determined using pig-to-non-human primate (NHP) corneal transplantation models. The infectivity of PERVs from the SNU miniature pig keratocytes was investigated by coculture with a human embryonic kidney cell line. Twenty-two rhesus macaques underwent xenocorneal transplantation as follows: (i) group 1 (n=4): anterior lamellar keratoplasty (LKP) with freshly preserved porcine corneas, (ii) group 2 (n=5): anterior LKP with decellularized porcine corneas followed by penetrating keratoplasty (PKP) with allografts, (iii) group 3 (n=3): PKP under steroid-based immunosuppression, (iv) group 4 (n=4): PKP under anti-CD154 antibody-based immunosuppression, (v) group 5 (n=4): deep anterior LKP with freshly preserved porcine corneas under anti-CD40 antibody-based immunosuppression, and (vi) group 6 (n=2): PKP under anti-CD40 antibody-based imm...Continue Reading

References

Mar 1, 1997·Nature Medicine·C PatienceR A Weiss
Mar 27, 2001·Transplant International : Official Journal of the European Society for Organ Transplantation·M LossU Martin
May 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thomas A EricssonClive Patience
Sep 6, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Markus IrgangJoachim Denner
Mar 17, 2004·Biomaterials·Klaus KallenbachHeike Mertsching
May 3, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Monica E WinklerUlrich Martin
Mar 3, 2009·Tissue Engineering. Part C, Methods·Joo Youn OhJin Hak Lee
May 27, 2009·Current Opinion in Organ Transplantation·Linda Scobie, Yasuhiro Takeuchi
Nov 26, 2010·Cornea·Hidetaka Hara, David K C Cooper
Jul 12, 2011·Investigative Ophthalmology & Visual Science·Hyuk Jin ChoiWon Ryang Wee
Apr 14, 2012·Xenotransplantation·Jay A FishmanYasuhiro Takeuchi
Aug 1, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M LoweK A Reimann
Aug 16, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda ScobieJean-Paul Soulillou
Jul 8, 2014·Laboratory animal research·Seongsoo HwangSeoki Im
Sep 2, 2014·Xenotransplantation·Mee Kum KimUNKNOWN International Xenotransplantation Association
Feb 14, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H J ChoiW R Wee
Oct 13, 2015·Science·Luhan YangGeorge Church
Nov 5, 2016·Virus Research·Vladimir A MorozovRobert Elliott

❮ Previous
Next ❯

Citations

Dec 7, 2017·Transplant International : Official Journal of the European Society for Organ Transplantation·Raphael P H MeierLeo H Buhler
Jun 23, 2018·Xenotransplantation·Latifah AminKhaidzir Ismail
Sep 12, 2017·Xenotransplantation·Mohammed Ezelerab, Christopher Burlak
Oct 30, 2019·Xenotransplantation·Abdulkadir IsidanBurcin Ekser
Aug 7, 2020·Progress in Retinal and Eye Research·Chang Ho YoonMee Kum Kim
Apr 26, 2021·The Ocular Surface·Ghasem YazdanpanahAli R Djalilian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.