Long-term self-renewable feeder-free human induced pluripotent stem cell-derived neural progenitors

Stem Cells and Development
Shiva NematiHossein Baharvand

Abstract

Human induced pluripotent stem cells (hiPSCs) have led to an important revolution in stem cell research and regenerative medicine. To create patient-specific neural progenitors (NPs), we have established a homogenous, expandable, and self-renewable population of multipotent NPs from hiPSCs, using an adherent system and defined medium supplemented with a combination of factors. The established hiPSC-NPs highly expressed Nestin and Sox1. These NPs were continuously propagated for ~1 year without losing their potential to generate astrocytes, oligodendrocytes, and functional neurons and maintained a stable chromosome number. Voltage clamp analysis revealed outward potassium currents in hiPSC-NPs. The self-renewal characteristic of the NPs was demonstrated by a symmetrical mode of Nestin-positive cell division. Additionally, these hiPSC-NPs can be easily frozen and thawed in the presence of Rho-associated kinase (ROCK) inhibitor without losing their proliferation, karyotype stability, and developmental potential. The characteristics of our generated hiPSC-NPs provide the opportunity to use patient-specific or ready-to-use hiPSC-NPs in future biomedical applications.

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Citations

Apr 6, 2013·Stem Cells and Development·Masood BazrgarHossein Baharvand
Jan 25, 2012·The Journal of Clinical Investigation·Christine VarelaNathalie Lefort
Jan 25, 2012·The Journal of Clinical Investigation·Neil J Harrison
Jul 22, 2011·Stem Cells International·Serpen DurnaogluKursad Genc
Aug 25, 2012·Stem Cells International·Joel Sng, Thomas Lufkin
Feb 22, 2012·Stem Cell Reviews and Reports·Yousof GheisariMohammad Vasei
Feb 11, 2015·Expert Review of Neurotherapeutics·Jeremy Micah CrookEva Tomaskovic-Crook
Jan 23, 2016·Stem Cells International·Shuling ZhouHasan X Avci
Aug 26, 2014·Journal of Biotechnology·Yu Wen, Sha Jin
Sep 5, 2014·Tissue Engineering. Part C, Methods·Sébastien SartYan Li
Oct 30, 2016·Biochemical and Biophysical Research Communications·Minoru AsadaHidenori Suzuki
Mar 16, 2019·Annals of the New York Academy of Sciences·M Catarina Silva, Stephen J Haggarty
Mar 30, 2019·Stem Cell Research & Therapy·Negin AfrangFatemeh Kouhkan
Apr 21, 2019·Journal of Cellular Physiology·Atiyeh MohammadshiraziSahar Kiani

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Methods Mentioned

BETA
flow cytometry
PCR
phase contrast
phase contrast microscopy
FCS

Software Mentioned

SPSS
pClamp10
Clampfit
Perlprimer

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