Long-term stability of endogenous B-type natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP) in frozen plasma samples

Clinical Chemistry and Laboratory Medicine : CCLM
Thomas MuellerMeinhard Haltmayer

Abstract

The aim of the present study was to assess the long-term stability of endogenous B-type natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP) in plasma samples stored at -20 degrees C without addition of protease inhibitors (e.g., aprotinin). Stability of BNP and NT-proBNP was tested in 60 EDTA plasma samples with BNP values between 30 and 420 pg/ml. Initial BNP and NT-proBNP plasma concentrations were determined within four hours after blood collection using the AxSYM BNP and the Elecsys NT-proBNP assays. Subsequently, all samples were stored at -20 degrees C and were thawed for the second BNP and NT-proBNP determination on the two instruments after one day, 30 days, 60 days, 90 days and 120 days, respectively. Mean recovery (i.e., residual immunoreactivity) of BNP and NT-proBNP expressed in percent of the initial value for the given time interval of storage was calculated. Mean recovery of BNP was less than 70% after one day of storage at -20 degrees C and decreased to less than 50% after two to four months of storage (e.g., recovery of endogenous BNP after three months of storage at -20 degrees C ranging from 0% to 71%). In contrast, mean recovery of NT-proBNP was generally greater than 90%, irrespective of the dur...Continue Reading

References

Sep 11, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·H ShimizuH Teraoka
Sep 15, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·A Gobinet-GeorgesL Bordenave
Sep 19, 2000·Italian heart journal. Supplement : official journal of the Italian Federation of Cardiology·R Latini, S Masson
Nov 26, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kiang-Teck J YeoAnthony W Butch

❮ Previous
Next ❯

Citations

Jun 17, 2008·International Journal of Legal Medicine·Katarzyna MichaudPatrice Mangin
Jun 11, 2005·Journal of Cardiac Failure·Kiang-Teck J YeoTimothy Brough
Feb 5, 2011·European Heart Journal·Kristian ThygesenUNKNOWN Study Group on Biomarkers in Cardiology of the ESC Working Group on Acute Cardiac Care
Feb 22, 2008·Journal of Veterinary Internal Medicine·D J ConnollyM Metcalf
Aug 28, 2009·Journal of Veterinary Internal Medicine·E RaffanJ Archer
Jun 10, 2010·BMC Medicine·Daniel Chan, Leong L Ng
Oct 20, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Francesca Di SerioNicola Pansini
May 9, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe BanfiGiuseppe Lippi
Sep 13, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Massimo Daves, Roberto Cemin
Jul 1, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Marta PereiraHenrique Barros
Dec 1, 2007·Biomarkers in Medicine·Wayne L MillerAllan S Jaffe
Feb 8, 2011·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·David J ConnollyHarriet M Syme
Mar 17, 2010·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·Stephen A CollinsJoanne D Harris
Apr 28, 2009·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·David J ConnollyHarriet M Syme
Feb 22, 2008·The American Journal of Cardiology·Jordi Ordonez-LlanosRobert H Christenson
Jan 27, 2007·European Journal of Heart Failure·Radka HazukováHana Strítecká
May 12, 2007·Clinical Endocrinology·Mikkel AndreassenLars Østergaard Kristensen
Dec 22, 2006·European Journal of Clinical Investigation·T WolberP Ammann
Jan 3, 2006·European Journal of Heart Failure·Antonio MuscariMarco Zoli
Feb 6, 2014·Journal of Veterinary Internal Medicine·K SjöstrandK Höglund
Oct 19, 2006·Journal of Cardiac Failure·Sohail Q KhanLeong L Ng
May 13, 2015·American Heart Journal·Alan H B Wu
Jul 16, 2013·Biochimica Et Biophysica Acta·Irina A Sergeeva, Vincent M Christoffels
Jul 17, 2007·Journal of the American College of Cardiology·Torbjørn OmlandUNKNOWN PEACE Investigators
May 5, 2009·International Journal of Cardiology·Oliver BruderPeter Hunold
Sep 11, 2016·Clinical Biochemistry·Jorge Andrés CarvajalAna María Delpiano
Feb 18, 2014·Asian Cardiovascular & Thoracic Annals·Alexander E Berezin, Tatiana A Samura
Jan 27, 2011·Korean Circulation Journal·Kyung-Hoon LeeByung-Su Yoo
Feb 26, 2015·Clinical Journal of the American Society of Nephrology : CJASN·Magid A FahimJonathan C Craig
Jul 31, 2019·Expert Review of Proteomics·Tamara TominRuth Birner-Gruenberger
Sep 12, 2007·Journal of Clinical Laboratory Analysis·Giuseppe LippiGian Cesare Guidi
Jul 23, 2005·Journal of Applied Physiology·Morten SchouNiels H Secher
May 17, 2008·Annals of Clinical Biochemistry·B CauliezA Lavoinne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Chemistry and Laboratory Medicine : CCLM
Lori J SokollChristiana Zingler
Clinica Chimica Acta; International Journal of Clinical Chemistry
Kiang-Teck J YeoAnthony W Butch
Clinical Chemistry and Laboratory Medicine : CCLM
A Gobinet-GeorgesL Bordenave
The New England Journal of Medicine
E R LevinW K Samson
© 2021 Meta ULC. All rights reserved