Long-term stabilization of polysaccharide electrospun fibres by in situ cross-linking

Journal of Biomaterials Science. Polymer Edition
Liya ShiSing Yian Chew

Abstract

Cross-linking of polysaccharide electrospun constructs using currently available techniques results in poor scaffold structural stability. In general, cross-linked substrates lose their nanofibrous architecture within a short time under physiological conditions. In this study, we introduce an in situ cross-linking electrospinning technique to fabricate and stabilize pullulan/dextran fibres. Pullulan/dextran (4:1 weight ratio, 16.7 and 20 wt%) solutions were preloaded with the chemical cross-linker, trisodium trimetaphosphate (STMP), to enable cross-linking during electrospinning. By increasing STMP from 4 to 16 wt%, the average diameter of electrospun fibres increased significantly from 268 ± 35 nm to 416 ± 74 nm (P < 0.05). Additionally, the enhanced cross-linking effectively decreased the swelling extent of the scaffolds. In particular, in the presence of 10 wt% gelatin, a significant decrease in scaffold swelling ratio was observed (208.5 ± 31.3% at 4 wt% STMP vs 133.1 ± 9.1% at 16 wt% STMP, P < 0.05). In vitro stability studies demonstrated the retention of scaffold fibrous morphology and negligible weight loss in all samples after 28 days. Environmental SEM analysis revealed that at least 16 wt% STMP was required in order ...Continue Reading

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Citations

May 24, 2014·Journal of Biomedical Materials Research. Part a·Xu JiangSing Yian Chew
Aug 11, 2015·Carbohydrate Polymers·Véronique WintgensCatherine Amiel
Apr 19, 2015·Trends in Biotechnology·Narendra ReddyQiuran Jiang

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