Long-term stabilized blood samples as controls for flow cytometric HLA-B27 screening: a feasibility study

Cytometry. Part B, Clinical Cytometry
Wilfried H B M LeveringDavid Barnett

Abstract

Long-term stabilized blood samples are potentially useful as positive or negative procedure controls for flow cytometric HLA-B27 screening, and could serve as test samples in an external quality assessment (EQA) scheme. We evaluated long-term stabilized whole blood specimens as prepared for the UK NEQAS for Leucocyte Immunophenotyping EQA scheme (Sheffield, UK). Peripheral blood samples were obtained from nine blood bank donors with known HLA-B typing. Short-term stabilization with Trans-FIXtrade mark was performed before shipment to Sheffield. Thereafter, long-term stabilization was performed. Commercially available HLA-B27 mAb were tested periodically between 1 week and 12 months on (i) fresh, (ii) short-term stabilized, and (iii) long-term stabilized blood samples using a stain, lyse, and wash technique. We compared the forward scatter (FSC), sideward scatter (SSC), and fluorescence signals of lymphocytes as a function of time. Furthermore, a pilot send-out with stabilized blood samples of four blood bank donors was distributed among the participants to the Benelux EQA scheme for HLA-B27 screening, and results were compared with historical EQA data obtained using nonstabilized blood samples from the same donors. There were n...Continue Reading

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Citations

Jun 24, 2015·Cytometry. Part B, Clinical Cytometry·Frank W M B PreijersUNKNOWN Section Immunological and Molecular Cell Diagnostics of the Foundation for Quality Assessment in Medical Laboratories(SKML)
Feb 25, 2018·Cytometry. Part B, Clinical Cytometry·Yang ZengKevin Judge
May 15, 2013·Annals of Laboratory Medicine·Bo Young Seo, Dong Il Won

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