Long-term survival and immunological tolerance of human epidermal allografts produced in culture

Transplantation
J ThivoletG Mauduit

Abstract

Human epidermal cells from a small skin specimen can be grown in culture into multilayered sheets suitable for the permanent coverage of large burn wounds when used as epidermal autografts. We report here on the long-term survival of such cultured epidermal sheets used as epidermal allografts (EAG) across a major histocompatibility barrier in three nonimmunosuppressed adult patients, suffering from large chronic grafted leg ulcers, where the EAG have been placed to cover the conventional split-thickness skin autograft donor site. The absence of rejection was based upon clinical, histological, and immunopathological observation of the allografted sites at various intervals after grafting of the EAG. The identity of the epidermal cells on the grafted area with cultured cells from allogeneic donor was then established after blood substance typing by indirect immunofluorescence. Furthermore, epidermal cells from cultured sheets, but not control human cells from freshly excised normal epidermis, failed to stimulate the recipient peripheral blood cells in the mixed epidermal cell lymphocyte culture reaction, a finding that is related to the complete absence of class-II-antigen-bearing cells in cultured epidermis. This absence of T ce...Continue Reading

Citations

Mar 1, 1991·Journal of Cellular Biochemistry·N L ParenteauE Bell
Jan 1, 1987·Archives of Dermatological Research·E TinoisD Schmitt
Jul 1, 1991·British Journal of Plastic Surgery·W F KaawachC R Rayner
Jul 1, 1992·British Journal of Plastic Surgery·J Nanchahal, C M Ward
Jul 1, 1991·Immunology Letters·J W Fabre
Jul 1, 1995·American Journal of Surgery·S MyersI Leigh
Oct 24, 1987·Lancet·T Phillips, O Kehinde
Jan 7, 1998·Clinical Therapeutics·W H Eaglstein, V Falanga
Jul 9, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Evert N LammeEsther Middelkoop
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E S FenjvesL B Taichman
Apr 1, 1993·The Journal of Experimental Medicine·A Bonomo, P Matzinger
Oct 1, 1995·Tissue Engineering·G G Gallico, N E O'Connor
Sep 15, 2004·Tissue Engineering·M GriffithsH Navsaria
Feb 15, 2012·Annals of Surgery·Giuseppe OrlandoShay Soker
Jan 1, 1990·The British Journal of Dermatology·R G TeepeB J Vermeer
Nov 1, 1990·Clinical and Experimental Dermatology·M ConyA Taïeb
Aug 1, 1993·The Journal of Dermatologic Surgery and Oncology·T J Phillips
Aug 29, 2012·PloS One·Wan Tai SeetBt Hj Idrus Ruszymah
Dec 11, 2002·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Sharon GohariJean-Christophe Lapiere
Apr 12, 2000·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Z Ruszczak, R A Schwartz
Apr 1, 1994·European Journal of Immunology·J Péguet-NavarroD Schmitt
Jul 5, 2005·Expert Opinion on Investigational Drugs·J Mansbridge
Jan 5, 2002·Expert Opinion on Biological Therapy·Jonathan Mansbridge
Jul 5, 2005·Expert Opinion on Investigational Drugs·J Mansbridge
Nov 16, 2010·The Surgical Clinics of North America·Markéta Límová
Apr 17, 2007·The Journal of Investigative Dermatology·Richard A F ClarkMarcia G Tonnesen
Jul 10, 2001·The Journal of Investigative Dermatology·A KernJ Mansbridge
Aug 1, 1990·Journal of the American Academy of Dermatology·T J PhillipsB A Gilchrest
Aug 1, 1989·Journal of the American Academy of Dermatology·T J PhillipsB A Gilchrest
May 24, 2008·Immunology·Rocío Isabel Domínguez-CastilloWalid Kuri-Harcuch
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jan Jeroen VranckxFeng Yao
Jun 4, 2002·British Journal of Plastic Surgery·I JonesR Martin
Jan 1, 1994·Clinics in Dermatology·T J Phillips

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.