Long-term survival of Streptococcus pyogenes in rich media is pH-dependent.

Microbiology
Dragutin J Savic, W Michael McShan

Abstract

The mechanisms that allow Streptococcus pyogenes to survive and persist in the human host, often in spite of antibiotic therapy, remain poorly characterized. Therefore, the determination of culture conditions for long-term studies is crucial to advancement in this field. Stationary cultures of S. pyogenes strain NZ131 and its spontaneous small-colony variant OK171 were found to survive in rich medium for less than 2 weeks, and this inability to survive resulted from the acidification of the medium to below pH 5.5, which the cells did not tolerate for longer than 6-7 days. The growth of NZ131 resulted in acidification of the culture to below pH 5.5 by the onset of stationary phase, and the loss of viability occurred in a linear fashion. These results were also found to be true for M49 strain CS101 and for M1 strain SF370. The S. pyogenes strains could be protected from killing by the addition of a buffer that stabilized the pH of the medium at pH 6.5, ensuring bacterial survival to at least 70 days. By contrast, increasing the glucose added to the medium accelerated the loss of culture viability in strain NZ131 but not OK171, suggesting that the small-colony variant is altered in glucose uptake or metabolism. Similarly, acidific...Continue Reading

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Sep 30, 2008·Journal of Bacteriology·W Michael McShanDragutin J Savic

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Citations

Jun 16, 2015·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Danielle McShanHongtao Yu
Apr 3, 2020·Current Microbiology·Leonhard MenschnerReinhard Berner
Mar 29, 2014·Journal of Food and Drug Analysis·Danielle McShanHongtao Yu
Jun 12, 2020·Archives of Microbiology·Elakshi DekaboruahAnil Kumar Verma

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