Long-term survival of transplanted stem cells in immunocompetent mice with muscular dystrophy.

The American Journal of Pathology
Gregory Q WallaceElizabeth M McNally

Abstract

Satellite cells refer to resident stem cells in muscle that are activated in response to damage or disease for the regeneration and repair of muscle fibers. The use of stem cell transplantation to treat muscular diseases has been limited by impaired donor cell survival attributed to rejection and an unavailable stem cell niche. We isolated a population of adult muscle mononuclear cells (AMMCs) from normal, strain-matched muscle and transplanted these cells into delta-sarcoglycan-null dystrophic mice. Distinct from other transplant studies, the recipient mice were immunocompetent with an intact endogenous satellite cell pool. We found that AMMCs were 35 times more efficient at restoring sarcoglycan compared with cultured myoblasts. Unlike cultured myoblasts, AMMC-derived muscle fibers expressed sarcoglycan protein throughout their entire length, consistent with enhanced migratory ability. We examined the capacity of single injections of AMMCs to provide long-term benefit for muscular dystrophy and found persistent regeneration after 6 months, consistent with augmentation of the endogenous stem cell pool. Interestingly, AMMCs were more effectively engrafted into aged dystrophic mice for the regeneration of large clusters of sarco...Continue Reading

References

Sep 20, 1991·Cell·J M Ervasti, K P Campbell
Aug 1, 1991·Journal of the Neurological Sciences·J M Zacharias, J E Anderson
Jan 1, 1991·Muscle & Nerve·S WakefordT A Partridge
Dec 1, 1973·Differentiation; Research in Biological Diversity·E M Pantelouris
Aug 1, 1971·The Anatomical Record·F P Moss, C P Leblond
Jan 1, 1982·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·F H Lubbe, O B Zaalberg
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H M BlauG K Pavlath
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G BulfieldK J Moore
Sep 28, 1995·The New England Journal of Medicine·J R MendellR Rice
Feb 1, 1995·Muscle & Nerve·J G QuinlanD L Denman
Apr 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A IrintchevA Wernig
Jul 1, 1993·Annals of Neurology·G KarpatiM Vanasse
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·N DeconinckJ M Gillis
Nov 1, 1996·Human Molecular Genetics·Y YangJ M Wilson
Nov 1, 1996·Human Molecular Genetics·E M McNallyE P Hoffman
Jul 1, 1996·Muscle & Nerve·Y FanM Grounds
Aug 26, 1998·The Journal of Clinical Investigation·W KuangE Engvall
Sep 10, 1998·The Journal of Cell Biology·Z QuJ Huard
Oct 1, 1998·Biochemical and Biophysical Research Communications·V BrusseeJ P Tremblay
Dec 21, 2000·The Journal of Cell Biology·J R BeauchampP S Zammit
Jun 18, 2002·Journal of Cellular and Molecular Medicine·G M SmytheM D Grounds
Jan 28, 2003·Muscle & Nerve·Terence A Partridge
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·A MAURO
Mar 27, 2004·Neuropathology and Applied Neurobiology·S H Laval, K M D Bushby
May 1, 2004·Circulation Research·Karen A LapidosElizabeth M McNally
Feb 24, 2005·Cell Cycle·Irina M Conboy, Thomas A Rando
Sep 6, 2005·Science·Didier MontarrasMargaret Buckingham
Sep 24, 2005·Cell Cycle·Charlotte A Collins, Terence A Partridge
Dec 29, 2005·The Journal of Cell Biology·Frédéric RelaixMargaret Buckingham

❮ Previous
Next ❯

Citations

Sep 29, 2009·Expert Reviews in Molecular Medicine·Dorianna Sandonà, Romeo Betto
Aug 15, 2009·The Journal of Trauma·Peter A WalkerCharles S Cox
Dec 31, 2010·Transplantation·Kathryn L Pellegrini, Manfred W Beilharz
Nov 12, 2010·Science Translational Medicine·John K HallBradley B Olwin
Sep 24, 2008·Annual Review of Physiology·Gregory Q Wallace, Elizabeth M McNally
Oct 27, 2009·Experimental Cell Research·Karlijn J WilschutBernard A J Roelen
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Amer MahmoodMoustapha Kassem
Dec 20, 2012·Experimental and Therapeutic Medicine·Xiangyi ZengWei Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Antibody Engineering

Antibody engineering technologies are constantly advancing to improve the clinical effectiveness of monoclonal and bispecific antibodies. Discover the latest research on Antibody Engineering here.