Long-term viability of transplanted hybrid cellular spheroids within chondrocyte sheets

Transplantation Proceedings
J I LeeY S Kim

Abstract

Encapsulation of transplanted cells within an immunoisolating membrane may provide a new strategy for protecting these cells from recipient immune responses without the use of immunosuppressive drugs. We have previously reported a novel concept of immunoisolation and immunodelusion using recipient cells instead of traditional artificial materials. We developed a chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas (CSI-BAP) that would enable transplantation of cells across allogeneic and xenogeneic barriers without the cells being recognized as donor cells and without the need for immunosuppression. Recently, we have constructed hybrid cellular spheroids (HCSs) containing cells from two different cell lines (RIN-5F, an insulin-secreting cell line, and Hep-G2, a hepatocellular carcinoma cell line) to enhance the function and biocompatibility of the HCSs. These HCSs were then encapsulated with multiple layers of chondrocyte sheets obtained from the auricular cartilage of Sprague-Dawley (SD) rats. The in vitro ability of the CSI-BAP to secrete insulin was tested before transplantation. Histological evaluation of CSI-BAP chondrocyte microencapsulated immunoisolated islet morphology and viability of allogeneic o...Continue Reading

References

Mar 1, 1981·The American Journal of Medicine·P E LacyE H Finke
Aug 16, 2000·Advanced Drug Delivery Reviews·B Ríhová
Jan 3, 2006·Diabetes Technology & Therapeutics·Seda KizilelEmmanuel Opara
May 13, 2008·Cell Transplantation·Jeong Ik LeeTakashi Kenmochi
Mar 3, 2009·Transplantation Proceedings·K H HuhY S Kim

❮ Previous
Next ❯

Citations

Jul 21, 2015·Tissue Engineering. Part B, Reviews·Daniel T BowersKenneth L Brayman

❮ Previous
Next ❯

Related Concepts

Related Feeds