Long transcripts from dinoflagellate chloroplast minicircles suggest "rolling circle" transcription.

The Journal of Biological Chemistry
Yunkun Dang, Beverley R Green

Abstract

The chloroplast genome of a dinoflagellate consists of a group of small circular DNA molecules (minicircles), most of which carry a single gene. With RT-PCR, primer extension, and Northern analyses, we show that the entire minicircle is transcribed and that some minicircles can produce RNAs larger than themselves. Using an RNA ligase-mediated rapid amplification of cDNA ends method, we were able to detect large processed precursors that are generated by endonucleolytic cleavage of an even longer molecule. This cleavage produces the mature mRNA 3'-end and at the same time the 5'-end of the precursor. The tRNAs encoded by the petD and psbE minicircles appear to be processed in the same way. We propose a "rolling circle" model for chloroplast transcription in which transcription would proceed continuously around the minicircular DNA to produce transcripts larger than the minicircle itself. These transcripts would be further processed into discrete mature mRNAs and tRNAs.

References

Feb 1, 1988·European Journal of Biochemistry·P Westhoff, R G Herrmann
Jul 17, 1999·Nature·Z ZhangT Cavalier-Smith
Mar 25, 2000·Molecular & General Genetics : MGG·A C Barbrook, C J Howe
Aug 18, 2000·Biochimie·R A MondeD B Stern
Mar 17, 2001·EMBO Reports·M Mörl, A Marchfelder
Jan 26, 2002·Molecular Genetics and Genomics : MGG·A C BarbrookC J Howe
May 29, 2002·The EMBO Journal·Steffen SchifferAnita Marchfelder
Feb 6, 2004·Current Biology : CB·Jeremiah D HackettDebashish Bhattacharya
Jul 30, 2005·The Journal of Biological Chemistry·María Ceballos-Chávez, Agustín Vioque
Sep 7, 2005·Photosynthesis Research·David L Herrin, Jöerg Nickelsen
Jan 26, 2006·Nucleic Acids Research·Yunling Wang, David Morse
Nov 25, 2006·BMC Genomics·Adrian C BarbrookChristopher J Howe
Mar 3, 2007·Molecular Genetics and Genomics : MGG·Monika Swiatecka-HagenbruchThomas Börner
Oct 16, 2007·Protist·R Ellen R NisbetChristopher J Howe
Apr 9, 2008·Physiological Reviews·Richard C Scarpulla
Feb 12, 2009·Nucleic Acids Research·Siu Kai Leung, Joseph T Y Wong
May 5, 2009·Plant Physiology·Giusy CaninoAnita Marchfelder
Jul 1, 1995·Journal of the American Chemical Society·Sarah L DaubendiekEric T Kool

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Citations

Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Richard G Dorrell, Christopher J Howe
Jan 24, 2014·PLoS Genetics·Richard G DorrellChristopher J Howe
Jun 2, 2014·Genome Biology and Evolution·Sutada MungpakdeeEiichi Shoguchi
Jun 21, 2011·Annual Review of Microbiology·Jennifer H Wisecaver, Jeremiah D Hackett
Apr 26, 2011·Research in Microbiology·Senjie Lin
Aug 4, 2016·Molecular and Biochemical Parasitology·R E R Nisbet, J L McKenzie
Apr 5, 2018·Genome Biology and Evolution·Christen M KlingerElisabeth Richardson
Jan 19, 2021·Journal of Phycology·Richard G Dorrell

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