Longikaurin E induces apoptosis of pancreatic cancer cells via modulation of the p38 and PI3K/AKT pathways by ROS

Naunyn-Schmiedeberg's Archives of Pharmacology
Hai-bo ChengChang-Liang Xu

Abstract

Pancreatic cancer is a devastating disease with a poor prognosis. It ranks as the fourth or fifth most common cancer in men and women and has the lowest 5-year survival rate. Therefore, there is an urgent need to develop novel therapeutic agents for pancreatic cancer. Longikaurin E (LE), which is derived from the traditional herbal medicine Rabdosia longituba, had been reported to have anti-proliferative and pro-apoptotic properties in several types of cancers. In this study, we investigated the cytotoxic properties of LE against pancreatic cancer cells and explored the mechanism behind the observed apoptosis. Pancreatic cancer cell lines cultured in the presence of LE exhibited dose- and time-dependent growth suppression by clone formation, methylthiazoltetrazolium assay, lactate dehydrogenase cytotoxicity assay, and fluorescence-activated cell sorting analysis, respectively. In addition, these culture conditions also induced the generation of cellular reactive oxygen species (ROS). In order to determine the mechanisms underlying LE-induced cytotoxicity, we used reverse transcription polymerase chain reaction and Western blot analysis in the pancreatic cancer cell line PANC1. The results showed that the expression of Bax was n...Continue Reading

References

Jan 27, 1997·International Journal of Cancer. Journal International Du Cancer·N KyprianouJ G Rhee
Jun 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H A BurrisD D Von Hoff
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Nov 4, 2000·Science·L ZhangB Vogelstein
Aug 18, 2001·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·H SauerJ Hescheler
Feb 4, 2003·Nature Cell Biology·Josef M Penninger, Guido Kroemer
Jul 26, 2003·Journal of Natural Products·David J NewmanKenneth M Snader
Sep 16, 2003·Journal of Cell Science·Simon WillisDavid C S Huang
Mar 31, 2004·Lancet·Donghui LiJames L Abbruzzese
Sep 15, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Caio M Rocha LimaLangdon L Miller
May 24, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C LouvetUNKNOWN GISCAD
Aug 13, 2005·Anti-cancer Drugs·Daniel Morgensztern, Howard L McLeod
Apr 6, 2006·Cancer Research·Gregory M HayesLaurence J Miller
Jul 5, 2006·Molecular Cancer Therapeutics·Elisa GiovannettiRomano Danesi
Aug 15, 2006·Seminars in Cancer Biology·Chi V DangFeng Li
Sep 28, 2006·Natural Product Reports·Han-Dong SunQuan-Bin Han
Apr 5, 2007·Nature Protocols·Nicolaas A P FrankenChris van Bree
Apr 25, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Malcolm J MooreUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Feb 9, 2008·Cell Death and Differentiation·E GiannoniP Chiarugi
May 14, 2008·Current Cancer Drug Targets·Amancio CarneroJuan F M Leal
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Jun 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Cheong J LeeDiane M Simeone
Jul 9, 2008·Translational Oncology·Cheong J LeeDiane M Simeone
Sep 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Diane M Simeone
Jan 5, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M A TemperoE Van Cutsem
Apr 26, 2011·Methods in Molecular Biology·Johan van MeerlooJacqueline Cloos
Jul 19, 2011·Developmental Cell·Jean-Claude Martinou, Richard J Youle
Jul 29, 2011·International Journal of Cancer. Journal International Du Cancer·Su-Ni TangRakesh K Srivastava
Oct 5, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel D Von HoffManuel Hidalgo
Oct 26, 2011·International Journal of Cancer. Journal International Du Cancer·Paul J Grippo, Eric P Sandgren
Mar 6, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dandan ZhaoXiaoguang Yu
May 26, 2012·Journal of Natural Products·Wei-Guang WangHan-Dong Sun
Oct 9, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Rohitas Deshmukh, Vishal Trivedi
Feb 1, 2013·Journal of Agricultural and Food Chemistry·Haifang XiaoXuebo Liu
Jul 17, 2013·CA: a Cancer Journal for Clinicians·Christopher L WolfgangRalph H Hruban

❮ Previous
Next ❯

Citations

Dec 10, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·Veronica FerrucciMassimo Zollo
Feb 18, 2016·Oxidative Medicine and Cellular Longevity·Lun ZhangZhenhua Ma
May 26, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Roland Seifert
Sep 22, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Stefanie KlenkeUlrich H Frey
Mar 19, 2016·Oncotarget·Ramadevi SubramaniRajkumar Lakshmanaswamy
Apr 23, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yun XiaJiaming Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.