Longitudinal analysis of the premature infant intestinal microbiome prior to necrotizing enterocolitis: a case-control study

PloS One
Yanjiao ZhouKatherine E Gregory

Abstract

Necrotizing enterocolitis (NEC) is an inflammatory disease of the newborn bowel, primarily affecting premature infants. Early intestinal colonization has been implicated in the pathogenesis of NEC. The objective of this prospective case-control study was to evaluate differences in the intestinal microbiota between infants who developed NEC and unaffected controls prior to disease onset. We conducted longitudinal analysis of the 16S rRNA genes of 312 samples obtained from 12 NEC cases and 26 age-matched controls with a median frequency of 7 samples per subject and median sampling interval of 3 days. We found that the microbiome undergoes dynamic development during the first two months of life with day of life being the major factor contributing to the colonization process. Depending on when the infant was diagnosed with NEC (i.e. early vs. late onset), the pattern of microbial progression was different for cases and controls. The difference in the microbiota was most overt in early onset NEC cases and controls. In proximity to NEC onset, the abundances of Clostridium sensu stricto from Clostridia class were significantly higher in early onset NEC subjects comparing to controls. In late onset NEC, Escherichia/Shigella among Gamma...Continue Reading

Associated Clinical Trials

References

Feb 1, 1986·Pediatric Clinics of North America·M C Walsh, R M Kliegman
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·N N NanthakumarW A Walker
May 29, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M HällströmO Tammela
Jun 18, 2004·Pediatric Research·Marie-France de la CochetiereJean-Christophe Roze
Oct 21, 2006·Paediatric and Perinatal Epidemiology·Robert C HolmanLawrence B Schonberger
Jun 28, 2007·PLoS Biology·Chana PalmerPatrick O Brown
Jul 6, 2007·Journal of Perinatology : Official Journal of the California Perinatal Association·P V GordonR Clark
Jul 1, 2008·The Journal of Infectious Diseases·Catherine J HunterNemani V Prasadarao
Apr 11, 2009·PLoS Computational Biology·James Robert WhiteMihai Pop
Sep 29, 2009·The Journal of Pediatrics·Maka MshvildadzeVolker Mai
Aug 25, 2010·Pediatrics·Barbara J StollUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
Dec 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael J MorowitzJillian F Banfield
Jan 21, 2011·The New England Journal of Medicine·Josef Neu, W Allan Walker
May 24, 2011·The Journal of Surgical Research·Claudia N EmamiNemani V Prasadarao
May 9, 2012·Archives of Disease in Childhood. Fetal and Neonatal Edition·Juliette C MadanPatricia L Hibberd
Jun 16, 2012·Nature·UNKNOWN Human Microbiome Project Consortium
Jun 16, 2012·Nature·Tanya YatsunenkoJeffrey I Gordon
Aug 21, 2012·PLoS Biology·Dirk GeversCurtis Huttenhower
Dec 14, 2012·Gut·Ana Elena Pérez-CobasAndrés Moya
Jan 16, 2013·Genome Biology·Yanjiao ZhouGeorge M Weinstock
Feb 1, 2013·Science·Michelle I SmithJeffrey I Gordon
Apr 25, 2013·Seminars in Pediatric Surgery·Anatoly GrishinHenri R Ford
May 9, 2013·Journal of Proteome Research·Aifric O'SullivanCarolyn M Slupsky
Oct 19, 2013·Journal of Perinatology : Official Journal of the California Perinatal Association·R WadhawanUNKNOWN NICHD Neonatal Research Network
Oct 31, 2013·MBio·Elizabeth K CostelloDavid A Relman
Mar 15, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Meiqian WengW Allan Walker
Jul 19, 2014·Cell·Julia K GoodrichRuth E Ley
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Patricio S La RosaPhillip I Tarr
Sep 5, 2014·Science Translational Medicine·Amir ArdeshirDennis J Hartigan-O'Connor

❮ Previous
Next ❯

Citations

Oct 24, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Nadim CassirBernard La Scola
Jan 21, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fardou H HeidaMarcus C de Goffau
Feb 9, 2016·Future Microbiology·Nadim CassirBernard La Scola
Dec 15, 2015·Journal of Pediatric Gastroenterology and Nutrition·Josef Neu
Dec 15, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Maureen W GroerW Allan Walker
Jan 11, 2016·Paediatric Drugs·Joseph B Cantey
Mar 14, 2016·Clinical Therapeutics·Suchitra K HouriganJohn E Niederhuber
Mar 17, 2016·Food & Function·Ashkan HashemiElena M Comelli
Nov 10, 2015·Advances in Neonatal Care : Official Journal of the National Association of Neonatal Nurses·Katherine E GregoryMatt Gregas
Jun 19, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marie-José Butel, Julio Aires
Dec 18, 2016·Seminars in Perinatology·Josef Neu, Mohan Pammi
Dec 3, 2016·The Journal of Pediatrics·Joseph B CanteyLea H Mallett
Feb 7, 2017·Gastroenterology Clinics of North America·Michael A SilvermanJeffrey S Gerber
Jan 21, 2018·Applied and Environmental Microbiology·Sophia Schönherr-HellecJulio Aires
Jun 1, 2016·Inflammatory Bowel Diseases·James J AshtonDavid W Cleary
Dec 8, 2017·NPJ Biofilms and Microbiomes·Ninalynn DaquiganJames Robert White
Aug 8, 2018·Emerging Microbes & Infections·Raymond Kiu, Lindsay J Hall
Nov 6, 2018·Current Pediatric Reviews·Catalina Bazacliu, Josef Neu
Sep 14, 2018·Microbiome·Thao T B HoAkhil Maheshwari
May 21, 2020·Journal of Perinatology : Official Journal of the California Perinatal Association·Ronald I ClymanNancy K Hills
Mar 1, 2018·Expert Review of Gastroenterology & Hepatology·Daniel Johannes Cornelis BerkhoutTimotheüs Gualtherus Jacob de Meij
Jul 1, 2017·The American Journal of Clinical Nutrition·Jean-Christophe RozéUNKNOWN EPIFLORE Study Group
May 10, 2018·Frontiers in Microbiology·Robin R Shields-CutlerDan Knights
Nov 29, 2017·The Journal of Antimicrobial Chemotherapy·Jon Widding FjalstadClaus Klingenberg
Aug 11, 2018·Frontiers in Microbiology·Xinyan ZhangNengjun Yi
May 18, 2020·Applied Microbiology and Biotechnology·Junfeng ZouJinao Duan
Nov 9, 2018·Frontiers in Microbiology·Yujian WangRuiliang Zhu
Nov 15, 2018·BMC Microbiology·Erica L PlummerUNKNOWN ProPrems Study Group
Jul 22, 2018·Scientific Reports·B RusconiP I Tarr

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

lmer4
vegan
Metastats
PERMANOVA
R

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.