Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine

Proceedings of the National Academy of Sciences of the United States of America
Anna Z WecLaura M Walker

Abstract

A comprehensive understanding of the development and evolution of human B cell responses induced by pathogen exposure will facilitate the design of next-generation vaccines. Here, we utilized a high-throughput single B cell cloning technology to longitudinally track the human B cell response to the yellow fever virus 17D (YFV-17D) vaccine. The early memory B cell (MBC) response was mediated by both classical immunoglobulin M (IgM) (IgM+CD27+) and switched immunoglobulin (swIg+) MBC populations; however, classical IgM MBCs waned rapidly, whereas swIg+ and atypical IgM+ and IgD+ MBCs were stable over time. Affinity maturation continued for 6 to 9 mo following vaccination, providing evidence for the persistence of germinal center activity long after the period of active viral replication in peripheral blood. Finally, a substantial fraction of the neutralizing antibody response was mediated by public clones that recognize a fusion loop-proximal antigenic site within domain II of the viral envelope glycoprotein. Overall, our findings provide a framework for understanding the dynamics and complexity of human B cell responses elicited by infection and vaccination.

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Citations

Aug 5, 2020·Nature Biomedical Engineering·Pavlo GilchukRobert H Carnahan
Nov 10, 2020·Immunology and Cell Biology·Marios Koutsakos, Ali H Ellebedy
Dec 15, 2020·Advanced Drug Delivery Reviews·Carlos Cruz-TeranSamuel K Lai
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Feb 21, 2021·Nature Communications·Adam K WheatleyStephen J Kent
Feb 25, 2021·Science Immunology·Mrunal SakharkarLaura M Walker
Feb 9, 2021·Annual Review of Immunology·Tyler J Ripperger, Deepta Bhattacharya
Apr 29, 2021·Human Vaccines & Immunotherapeutics·Amandine BovayDaniel E Speiser
Jul 3, 2021·Vaccines·Jolynne MokayaGeorge M Warimwe
Jul 30, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·John Tyler SandbergHans-Gustaf Ljunggren
Sep 28, 2021·Frontiers in Immunology·Larance RonsardDaniel Lingwood

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Methods Mentioned

BETA
ELISA
PCR
enzyme-linked immunosorbent assay
biolayer
flow cytometry
surface plasmon resonance

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