Longitudinal in vivo imaging of retinal gliosis in a diabetic mouse model

Experimental Eye Research
Saravana Kumar, Lang Zhuo

Abstract

In this study, we visualize and quantify retinal gliosis in vivo for monitoring early diabetic retinopathy (DR) in a transgenic mouse model. Onset of diabetes was triggered via intraperitoneal injection of streptozotocin (STZ) into transgenic F1 hybrid (FVB/N × C57BL/6J) mice expressing green fluorescent protein (GFP) under the control of glial fibrillary acidic protein (GFAP) promoter. Retinal glial cells are imaged once pre-STZ treatment followed by weekly post-STZ imaging for five weeks using a confocal scanning laser ophthalmoscope. Mice develop diabetes one week after STZ induction as confirmed from the high blood glucose levels (>13.9 mmol/L). A significant increase is observed in the GFAP-GFP transgene expression from astrocytic cell bodies and processes as early as week 5 for the STZ-treated mice. Retinal astrocytes also undergo hyperplasia progressively from week 0 to 5. This precedes any structural abnormalities to the retinal vasculature. Immunohistochemistry (IHC) on retinal sections as well as quantitative RT-PCR of endogenous and transgene GFAP mRNA supports our in vivo observation. Our in vivo data correlates with clinical reports with regards to retinal gliosis-related inflammatory response during early diabetic...Continue Reading

References

Jul 1, 1997·Developmental Biology·L ZhuoA Messing
Mar 31, 1998·Diabetes·M MizutaniM Lorenzi
May 10, 2001·Clinical & Experimental Ophthalmology·E LiethUNKNOWN Penn State Retina Research Group
Aug 18, 2001·Diabetologia·M Lorenzi, C Gerhardinger
Jan 1, 2003·Survey of Ophthalmology·Thomas W GardnerSteven W Levison
May 26, 2005·Investigative Ophthalmology & Visual Science·Alistair J BarberSarah K Bronson
Aug 27, 2005·Investigative Ophthalmology & Visual Science·Matthew R RitterMartin Friedlander
Oct 27, 2005·Investigative Ophthalmology & Visual Science·Rachel A Feit-LeichmanDong Feng Chen
Jul 15, 2006·Progress in Retinal and Eye Research·Andreas BringmannAndreas Reichenbach
Aug 29, 2006·Diabetes·David A AntonettiUNKNOWN JDRF Diabetic Retinopathy Center Group
Feb 20, 2007·Optics Letters·David P BissCharles P Lin
Nov 30, 2007·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Erica L FletcherKate M Hatzopoulos
Jul 22, 2008·Arquivos brasileiros de oftalmologia·Pedro Durães SerrarbassaMarcio Fragoso Vieira
Jan 20, 2009·Investigative Ophthalmology & Visual Science·Gideon HoLang Zhuo

❮ Previous
Next ❯

Citations

Nov 29, 2013·Journal of Diabetes Research·Angela Ka Wai Lai, Amy C Y Lo
Feb 18, 2014·Survey of Ophthalmology·J Emanuel Ramos de CarvalhoReinier O Schlingemann
Oct 2, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Mário Cesar do Nascimento BevilaquaKarin C Calaza
Sep 29, 2011·PloS One·Meghana TareAmit Singh
Mar 4, 2020·International Journal of Molecular Sciences·Claudia G FrestaClaudio Bucolo
Aug 25, 2017·Current Diabetes Reports·Ana Maria OlivaresNeena Haider
Jun 28, 2019·Journal of Clinical Medicine·Todd McLaughlinSarah X Zhang
Apr 13, 2021·The Keio Journal of Medicine·Deokho LeeToshihide Kurihara
Jun 8, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ying QiQingqing Cui

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.