Longitudinal Metabolomics Profiling of Parkinson's Disease-Related α-Synuclein A53T Transgenic Mice

PloS One
Xi ChenHuaibin Cai

Abstract

Metabolic homeostasis is critical for all biological processes in the brain. The metabolites are considered the best indicators of cell states and their rapid fluxes are extremely sensitive to cellular changes. While there are a few studies on the metabolomics of Parkinson's disease, it lacks longitudinal studies of the brain metabolic pathways affected by aging and the disease. Using ultra-high performance liquid chromatography and tandem mass spectroscopy (UPLC/MS), we generated the metabolomics profiling data from the brains of young and aged male PD-related α-synuclein A53T transgenic mice as well as the age- and gender-matched non-transgenic (nTg) controls. Principal component and unsupervised hierarchical clustering analyses identified distinctive metabolites influenced by aging and the A53T mutation. The following metabolite set enrichment classification revealed the alanine metabolism, redox and acetyl-CoA biosynthesis pathways were substantially disturbed in the aged mouse brains regardless of the genotypes, suggesting that aging plays a more prominent role in the alterations of brain metabolism. Further examination showed that the interaction effect of aging and genotype only disturbed the guanosine levels. The young ...Continue Reading

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Citations

Jun 3, 2016·Frontiers in Physiology·Catherine G VasilopoulouMaria I Klapa
Oct 5, 2016·Aging and Disease·Débora LanznasterCarla I Tasca
May 23, 2017·PloS One·Seyed Ali GoldansazDavid S Wishart
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Jul 7, 2021·Expert Review of Proteomics·Nimisha GuptaSaima Wajid
Dec 10, 2020·Current Protein & Peptide Science·Debanjan Kundu, Vikash Kumar Dubey

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Methods Mentioned

BETA
metabolomics profiling
Magnetic

Software Mentioned

Metabolome
LIMS
Metabolon
Random Forests
R package
MetaboAnalyst
R

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